Electropolymerized Poly(3,4-ethylendioxythiophene)/Graphene Composite Film and its Application in Quantum Dots Electrochemiluminescence Immunoassay

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• 作者：Weiping Li (1)
  Weijian Dai (1)
  Lei Ge (1)
  Shenguang Ge (1)
  Mei Yan (1)
  Jinghua Yu (1)
  
• 关键词：Poly(3 ; 4 ; ethylendioxythiophene)/graphene ; CdTe quantum dots coated carbon nanosphere ; Electrochemiluminescence immunosensor ; Prostate ; specific antigen ; Point ; of care testing
• 刊名：Journal of Inorganic and Organometallic Polymers and Materials
• 出版年：2013
• 出版时间：May 2013
• 年：2013
• 卷：23
• 期：3
• 页码：719-725
• 全文大小：548 KB
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• 作者单位：Weiping Li (1)
  Weijian Dai (1)
  Lei Ge (1)
  Shenguang Ge (1)
  Mei Yan (1)
  Jinghua Yu (1)
  
  1. Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, People’s Republic of China
  
• ISSN：1574-1451

文摘

Poly(3,4-ethylendioxythiophene) (PEDOT) films have been electrochemically polymerized on graphene (GR) modified glassy carbon electrode (GCE) simply by a one-step cyclic voltammetry (CV) method in an aqueous media in the absence of any surfactant. The PEDOT/GR composite films were characterized by scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). This composite film provided an excellent platform for immobilization of antibody with good bioactivity, and conductivity through Au nanoparticles (AuNPs) as a linker. With a sandwich-type immunoassay format, the analyte and then the CdTe quantum dots coated carbon nanosphere (CNS@CdTe)-labeled antibody were successively bound to the immunosensor. The dual-amplification strategy greatly enhanced the sensitivity for point-of-care testing (POCT) of prostate-specific antigen (PSA). Under optimal conditions, the proposed method could detect PSA with a linear range of 0.001-0?ng?mL? and a detection limit down to 0.8?pg?mL?. The assay results of PSA in clinical serum samples from the Tumor Hospital were in acceptable agreement with the reference values from enzyme-linked immunosorbent assay. The proposed ECL immunosensor exhibited high sensitivity and stability, and could be applied in POCT of other tumor markers for remote regions and developing countries.