Affinity capillary electrophoresis: the theory of electromigration
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- 作者：Pavel Dubský ; Martin Dvořák ; Martin Ansorge
- 关键词：Capillary electrophoresis/electrophoresis ; Affinity capillary electrophoresis ; Electrokinetic chromatography ; Viscosity ; Ionic strength ; Separations/theory
- 刊名：Analytical and Bioanalytical Chemistry
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：408
- 期：30
- 页码：8623-8641
- 全文大小：
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Analytical Chemistry; Biochemistry, general; Laboratory Medicine; Characterization and Evaluation of Materials; Food Science; Monitoring/Environmental Analysis;
- 出版者：Springer Berlin Heidelberg
- ISSN：1618-2650
- 卷排序：408
文摘
We focus on the state-of-the-art theory of electromigration under single and multiple complexation equilibrium. Only 1:1 complexation stoichiometry is discussed because of its unique status in the field of affinity capillary electrophoresis (ACE). First, we summarize the formulas for the effective mobility in various ACE systems as they appeared since the pioneering days in 1992 up to the most recent theories till 2015. Disturbing phenomena that do not alter the mobility of the analyte directly but cause an unexpected peak broadening have been studied only recently and are also discussed in this paper. Second, we turn our attention to the viscosity effects in ACE. Change in the background electrolyte viscosity is unavoidable in ACE but numerous observations scattered throughout the literature have not been reviewed previously. This leads to an uncritical employment of correction factors that may or may not be appropriate in practice. Finally, we consider the ionic strength effects in ACE, too. Limitations of the current theories are also discussed and the tasks identified where open problems still prevail.