Comparison of the in vitro and in vivo toxic effects of three sizes of zinc oxide (ZnO) particles using flounder gill (FG) cells and zebrafish embryos

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• 作者：Li Han ; Yanan Zhai ; Yang Liu ; Linhua Hao…
• 关键词：Key wordszinc oxide (ZnO) ; nanoparticles ; cytotoxicity ; embryotoxicity ; zebrafish ; fish cells
• 刊名：Journal of Ocean University of China
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：16
• 期：1
• 页码：93-106
• 全文大小：
• 刊物主题：Oceanography; Meteorology;
• 出版者：Science Press
• ISSN：1993-5021
• 卷排序：16

文摘

Nano-sized zinc oxide (nZnO) particles are one kind of the most commonly used metal oxide nanoparticles (NPs). This study compared the cytotoxic and embryotoxic effects of three increasing sized ZnO particles (ϕ 30 nm, 80-150 nm and 2 μm) in the flounder gill (FG) cells and zebrafish embryos, and analyzed the contribution of size, agglomeration and released Zn2+ to the toxic effects. All the tested ZnO particles were found to be highly toxic to both FG cells and zebrafish embryos. They induced growth inhibition, LDH release, morphological changes and apoptosis in FG cells in a concentration-, size- and time-dependent manner. Moreover, the release of LDH from the exposed FG cells into the medium occurred before the observable morphological changes happened. The ultrasonication treatment and addition of serum favored the dispersion of ZnO particles and alleviated the agglomeration, thus significantly increased the corresponding cytotoxicity. The released Zn2+ ions from ZnO particles into the extracellular medium only partially contributed to the cytotoxicity. All the three sizes of ZnO particles tested induced developmental malformations, decrease of hatching rates and lethality in zebrafish embryos, but size- and concentration- dependent toxic effects were not so obvious as in FG cells possibly due to the easy aggregation of ZnO particles in freshwater. In conclusion, both FG cells and zebrafish embryos are sensitive bioassay systems for safety assessment of ZnO particles and the environmental release of ZnO particles should be closely monitored as far as the safety of aquatic organisms is concerned.