Carbamoylases: characteristics and applications in biotechnological processes

详细信息    查看全文

• 作者：Sergio Mart¨ªnez-Rodr¨ªguez ; Ana Isabel Mart¨ªnez-G¨®mez ; Felipe Rodr¨ªguez-Vico ; Josefa Mar¨ªa Clemente-Jim¨¦nez and Francisco Javier Las Heras-V¨¢zquez
• 关键词：l ; Carbamoylase ; N ; Carbamoyl ; l ; amino acid amidohydrolase ; d ; Carbamoylase ; N ; Carbamoyl ; d ; amino acid amidohydrolase ; Hydantoinase Process ; Ureidopropionase ; Peptidase family M20/M25/M40 ; Nitrilase family ; Kinetic resolution
• 刊名：Applied Microbiology and Biotechnology
• 出版年：2010
• 出版时间：January, 2010
• 年：2010
• 卷：85
• 期：3
• 页码：441-458
• 全文大小：871.1 KB

文摘

Enzymatic kinetic resolution is a widely used biotechnological tool for the production of enantiomerically pure/enriched compounds. This technique takes advantage of the enantioselectivity or enantiospecificity of an enzyme for one of the enantiomers of a racemic substrate to isolate the desired isomer. N-Carbamoyl-d- and l-amino acid amidohydrolases (d- and l-carbamoylases) are model enzymes for this procedure due to their strict enantiospecificity. Carbamoylase-based kinetic resolution of amino acids has been applied for the last three decades, allowing the production of optically pure d- or l-amino acids. Furthermore, this enzyme has become crucial in the industrially used multienzymatic system known as “Hydantoinase Process,” where the kinetic resolution produced by coupling an enantioselective hydantoinase and the enantiospecific carbamoylase is enhanced by the enzymatic/chemical dynamic kinetic resolution of the low-rate hydrolyzed substrate. This review outlines the properties of d- and l-carbamoylases, emphasizing their biochemical/structural characteristics and their biotechnological applications. It also pinpoints new applications for the exploitation of carbamoylases over the forthcoming years.