Phylogenetic analysis of PR genes in some pome fruit species with the emphasis on transcriptional analysis and ROS response under Erwinia amylovora inoculation in apple
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- 作者：Maryam Hassani ; Seyed Alireza Salami ; Jaber Nasiri ; Hamid Abdollahi…
- 关键词：Pome fruit species ; Erwinia amylovora ; Fire blight ; PR genes ; ROS response
- 刊名：Genetica
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：144
- 期：1
- 页码：9-22
- 全文大小：3,075 KB
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Seyed Alireza Salami (2)
Jaber Nasiri (3)
Hamid Abdollahi (4)
Zahra Ghahremani (1)
1. Department of Biotechnology, Faculty of Agriculture and Natural Resources, Azad University (Science and Research Branch), P.O. Box 4933, Tehran, 14155, Iran
2. Department of Horticultural Sciences, Faculty of Agriculture and Natural Resources, University of Tehran, P.O. Box 4111, Karaj, 31587, Iran
3. Department of Agronomy and Plant Breeding, Division of Molecular Plant Genetics, College of Agricultural & Natural Resources, University of Tehran, Karaj, Tehran, Iran
4. Department of Horticulture Research, Seed and Plant Improvement Institute, P.O. Box 4119, Karaj, 31585, Iran - 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Life Sciences
Animal Genetics and Genomics
Plant Genetics and Genomics
Human Genetics
Microbial Genetics and Genomics - 出版者：Springer Netherlands
- ISSN：1573-6857
文摘
Attempts were made to identify eight pathogenesis related (PR) genes (i.e., PR-1a, PR3-ch1, PR3-Ch2, PR3-Ch3, PR3-Ch4, PR3-Ch5, PR-5 and PR-8) from 27 genotypes of apple, quince and pear, which are induced in response to inoculation with the pathogen Erwinia amylovora, the causal agent of fire blight. Totally, 32 PR genes of different families were obtained, excepting PR3-Ch2 (amplified only in apple) and PR3-Ch4 (amplified only in apple and pear), the others were successfully amplified in all the genotypes of apple, quince and pear. Evolutionary, the genes of each family exhibited significant homology with each other, as the corresponded phylogenetic neighbor-joining-based dendrograms were taken into consideration. Meanwhile, according to the expression assay, it was deduced that the pathogen activity can significantly affect the expression levels of some selected PR genes of PR3-Ch2, PR3-Ch4, PR3-Ch5 and particularly Cat I in both resistant (MM-111) and semi-susceptible (MM-106) apple rootstocks. Lastly, it was concluded that the pathogen E. amylovora is able to stimulate ROS response, particularly using generation of hydrogen peroxide (H2O2) in both aforementioned apple rootstock. Keywords Pome fruit species Erwinia amylovora Fire blight PR genes ROS response