Microbial Conversion of Arachidonic Acid to Arachidonyl Alcohol by a New Acinetobacter Species
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- 作者：Toshihiro Nagao (1) nagao@omtri.or.jp
Yomi Watanabe (1)
Shigemitsu Tanaka (1)
Motohiro Shizuma (1)
Yuji Shimada (2) - 关键词：Acinetobacter sp. – ; Arachidonic acid – ; Arachidonyl alcohol – ; Fatty alcohol – ; Microbial conversion – ; Reduction – ; Wax ester
- 刊名：Journal of the American Oil Chemists' Society
- 出版年：2012
- 出版时间：September 2012
- 年：2012
- 卷：89
- 期：9
- 页码：1663-1671
- 全文大小：328.8 KB
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- ISSN：1558-9331
文摘
Arachidonyl alcohol rarely occurs in natural oils. It can be used as a substrate for production of several ether lipids possessing beneficial functions. Although arachidonyl alcohol has been produced on a laboratory scale by the chemical reduction of arachidonic acid, it will be difficult to scale up this process for industrial application. The aim of this study was to develop a new bioprocess for converting arachidonic acid to arachidonyl alcohol. Screening was conducted using 11 wax ester- (esters of fatty acids and fatty alcohols) producing strains reported in our previous study, and a single-cell oil containing arachidonic acid. A new strain, Acinetobacter species N-476-2, most effectively converted arachidonic acid to arachidonyl alcohol, which accumulated inside the cells as a wax ester. GC–MS, FT–IR, and NMR analyses showed that this strain reduced the carboxyl group of 5-cis,8-cis,11-cis,14-cis-arachidonic acid to a hydroxyl group without altering the position or configuration of the double bonds; the product was identified as 5-cis,8-cis,11-cis,14-cis-arachidonyl alcohol. A time-course study of cultivation showed that the amount of arachidonyl alcohol produced by the strain after 4 days was 2.2 mg/mL culture. The bioprocess using Acinetobacter sp. N-476-2 can be applied to the large-scale production of arachidonyl alcohol.