Changes in the respiratory microbiome during acute exacerbations of idiopathic pulmonary fibrosis
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- 作者：Philip L. Molyneaux ; Michael J Cox ; Athol U. Wells ; Ho Cheol Kim…
- 关键词：Idiopathic pulmonary fibrosis ; Acute exacerbation ; Bacterial infection ; 16S
- 刊名：Respiratory Research
- 出版年：2017
- 出版时间：December 2017
- 年：2017
- 卷：18
- 期：1
- 全文大小：451KB
- 刊物主题：Pneumology/Respiratory System;
- 出版者：BioMed Central
- ISSN：1465-993X
- 卷排序：18
文摘
Acute exacerbations of idiopathic pulmonary fibrosis (AE-IPF) have been defined as events of clinically significant respiratory deterioration with an unidentifiable cause. They carry a significant mortality and morbidity and while their exact pathogenesis remains unclear, the possibility remains that hidden infection may play a role. The aim of this pilot study was to determine whether changes in the respiratory microbiota occur during an AE-IPF. Bacterial DNA was extracted from bronchoalveolar lavage from patients with stable IPF and those experiencing an AE-IPF. A hyper-variable region of the 16S ribosomal RNA gene (16S rRNA) was amplified, quantified and pyrosequenced. Culture independent techniques demonstrate AE-IPF is associated with an increased BAL bacterial burden compared to stable disease and highlight shifts in the composition of the respiratory microbiota during an AE-IPF.