Molecular cloning and expression analysis of Relish gene from the ridgetail white prawn Exopalaemon carinicauda

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• 作者：Qianqian Ge ; Junping Liang ; Jitao Li ; Jian Li ; Yafei Duan ; Fazhen Zhao…
• 关键词：Exopalaemon carinicauda ; Relish ; Cloning ; Vibrio anguillarum ; WSSV ; Ammonia ; N stress ; Expression
• 刊名：Fisheries Science
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：81
• 期：4
• 页码：699-711
• 全文大小：2,002 KB
• 参考文献：1.Liu RY (1957) Palaemon and Macrobrachium. Bull Biol 6:14-3
  2.Xu WJ, Xie JJ, Shi H (2010) Hematodinium infections in cultured ridgetail white prawns, Exopalaemon carinicauda, in eastern China. Aquaculture 300:25-1View Article
  3.Xia DQ (1999) Biological characteristics and aquaculture technique of Exopalaemon carinicauda. China Fish 3:42-3 (in Chinese)
  4.Lightner DV, Revdman RM (1998) Shrimp diseases and current diagnostic methods. Aquaculture 164:201-20View Article
  5.Li GY (1995) Review of disease and immune mechanism in Penaeus chinensis. Marine Sciences 4:1- (in Chinese)
  6.Le Moullac G, Taravao T, Fran?aise P (2000) Environmental factors affect immune response and resistance in Crustaceans. The Advocate 18?9
  7.Aguirre-Guzman G, Sanchez-Martinez JG, Campa-Cordova AI (2009) Penaeid shrimp immune system. Thai J Vet Med 39:205-15
  8.Amparyup P, Kondo H, Hirono I, Aoki T, Tassanakajon A (2008) Molecular cloning, genomic organization and recombinant expression of a crustin-like antimi-crobial peptide from black tiger shrimp Penaeus monodon. Mol Immunol 45:1085-093PubMed View Article
  9.Hoffmann JA, Reichhart JM (2002) Drosophila innate immunity: an evolutionary perspective. Nat Immunol 3:121-26PubMed View Article
  10.Kush RS, Leulier F, Lemaitre B (2001) Drosophila immunity: two paths to NF-kB. Trends Immunol 22:260-64View Article
  11.De Gregorio E, Spellman PT, Tzou P (2002) The Toll and Imd pathways are the major regulators of the immune response in Drosophila. EMBO J 21:2568-579PubMed Central PubMed View Article
  12.Georgel P, Naitza S, Kappler C, Ferrandon D, Zachary D, Swimmer C et al (2001) Drosophila immune deficiency (IMD) is a death domain protein that activates antibacterial defense and can promote apoptosis. Dev cell 1:503-14PubMed View Article
  13.Lemaitre B, Kromer-Metzger E, Michaut L, Nicolas E, Meister M, Georgel P et al (1995) A recessive mutation, immune deficiency (IMD), defines two distinct control pathways in the Drosophila host defense. Proc Natl Acad Sci USA 92:9465-469PubMed Central PubMed View Article
  14.Naitza S, Rossé C, Kappler C, Georgel P, Belvin M, Gubb D et al (2002) The Drosophila immune defense against gram-negative infection requires the death protein dFADD. Immunity 17:575-81PubMed View Article
  15.Rutschmann S, Kilinc A, Ferrandon D (2002) Cutting edge: the Toll pathway is required for resistance to gram-positive bacterial infections in Drosophila. Immunology 168:1542-546View Article
  16.Hedengren M, Asling B, Dushay MS (1999) Relish, a central factor in the control of humoral but not cellular immunity in Drosophila. Mol Cell 4:827-37PubMed View Article
  17.Wang DN, Liu JW, Yang GZ, Zhang WJ, Wu XF (2002) Cloning of anti-LPS factor cDNA from Tachypleus tridentatus, expression in Bombyx mori larvae and its biological activity in vitro. Mol Biotechnol 21:1-PubMed View Article
  18.Belvin MP, Anderson KV (1996) A conserved signaling pathway: the Drosophila Toll-Dorsal pathway. Annu Rev Cell Dev Biol 12:393-16PubMed View Article
  19.Lemaitre B, Reichhart JM, Hoffmann JA (1997) Drosophila host defense: differential induction of antimicrobial peptide genes after infection by various classes of microorganisms. PNAS 94:14614-4619PubMed Central PubMed View Article
  20.Rutschmann S, Jung AC, Hetru C, Reichhart JM, Hoffmann JA, Ferrandon D (2000) The Rel protein DIF mediates the antifungal but not the antibacterial host defense in Drosophila. Immunity 12:569-80PubMed View Article
  21.Kang CJ, Wang JX, Zhao XF, Yang XM, Shao HL, Xiang JH (2004) Molecular cloning and expression analysis of the ch-penaedin, an antimicrobial peptide from Chinese shrimp, Fenneropenaeus chinensis. Fish Shellfish Immunol 16:513-25PubMed View Article
  22.Cuthbertson B, Bullesbach E, Bachere E, Fievet J, Gross P (2004) A new class (penaeidin class 4) of antimicrobial peptides from the Atlantic white shrimp (Litopenaeus setiferus) exhibits target specificity and an independent proline-rich-domain function. Biochem J 381:79-6PubMed Central PubMed View Article
  23.Visetnan S, Supungul P, Hirono I, Tassanakajon A, Rimphanitchayakit V (2015) Activation of Pm Relish from Penaeus monodon by yellow head virus. Fish Shellfish Immunol 42:335-44PubMed View Article
  24.Hoffmann JA, Reichhart JM, Hetru C (1995) Innate immunity in insects. Curr Opin Immunol 7:4-0PubMed View Article
  25.Shin SW, Kokoza V, Ahmed A, Raikhel AS (2002) Characterization of three alternatively spliced isoforms of the Rel/NF-κB transcription factor Relish from the mosquito Aedes aegypti. PNAS 99:9978-983PubMed Central PubMed View Article
  26.Meister S, Kanzok SM, Zheng XL, Luna C, Li TR, Hoa NT (2005) Immune signaling pathways regulating bacterial and malaria parasite infection of the mosquito Anopheles gambiae. Proc Natl Acad Sci USA 102:11420-1425PubMed Central PubMed View Article
  27.Liu WJ, Yang LS, Li XL (2013) Cloning and mRNA expression
• 作者单位：Qianqian Ge (1) (2)
  Junping Liang (3)
  Jitao Li (2)
  Jian Li (2)
  Yafei Duan (4)
  Fazhen Zhao (2)
  Hai Ren (2)
  
  1. Fisheries College, Ocean University of China, Qingdao, 266003, People’s Republic of China
  2. Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, People’s Republic of China
  3. College of Fisheries, Henan Normal University, Xinxiang, 453007, People’s Republic of China
  4. Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, People’s Republic of China
  
• 刊物主题：Fish & Wildlife Biology & Management; Freshwater & Marine Ecology; Food Science;
• 出版者：Springer Japan
• ISSN：1444-2906

文摘

Rel/NF-κB transcription factors play critical roles in induction and regulation of innate immune response in organisms. In this study, the full length of a Relish homolog cDNA from Exopalaemon carinicauda named EcRelish was 2141?bp encoding a 660 amino-acid polypeptide. EcRelish cDNA contained a conserved Rel homology domain and two nucleus localization signals. Sequence analysis indicated that the deduced amino acid sequence of the EcRelish showed high similarities to that of other crustaceans. Real time RT-PCR analysis showed that EcRelish mRNA expressed with different levels in tested tissues, and the highest expression was observed in the hemocytes. With longer infection time, the cumulative mortality rates increased gradually followed by the proliferation of Vibrio anguillarum and WSSV. The expression profiles of EcRelish gene were analyzed after V. a nguillarum, white spot syndrome virus (WSSV) challenge, and ammonia-N stress. The results showed that the expression levels of EcRelish mRNA in the hemocytes were up-regulated at 1-4?h after V. a nguillarum challenge. Meanwhile, the expression levels of EcRelish mRNA were up-regulated at 3?h after WSSV challenge. The expression of EcRelish in hemocytes was down-regulated significantly under ammonia-N stress during the experimental time. The results indicated that EcRelish might be involved in immune defense against pathogens and ammonia-N stress in E. c arinicauda.