Molecular characterization and different expression patterns of the FABP gene family during goat skeletal muscle development

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• 作者：Linjie Wang ; Li Li ; Jing Jiang ; Yan Wang ; Tao Zhong ; Yu Chen…
• 关键词：FABP ; Goat ; Skeletal muscle ; Different expression ; Intramuscular fat content (IMF)
• 刊名：Molecular Biology Reports
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：42
• 期：1
• 页码：201-207
• 全文大小：605 KB
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  17. Li L, Ji
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Animal Anatomy, Morphology and Histology
  Animal Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-4978

文摘

The FABP (adipocyte fatty acid-binding protein) genes play an important role in intracellular fatty acid transport and considered to be candidate genes for fatness traits in domestic animal. In this study, we cloned the cDNA sequences of goat FABP family genes and their expression patterns were detected by semi-quantitative RT-PCR and quantitative real time RT-PCR. Expression analysis showed that goat FABP1 gene was predominantly expressed in liver, kidney and large intestine. While FABP4 was widely expressed in many tissues with a high expression level was observed in the fat, skeletal muscle, stomach and lung. Notably, FABP2 gene was expressed specifically in small intestine. Moreover, goat FABP3 was expressed at 60 day with the highest level, then significantly (p?longissimus dorsi muscles among 3?day, 30?day and 60?day. Goat FABP4 was expressed at 3?day with the lowest level, then significantly (p?FABP3 mRNA expression levels and intramuscular fat (IMF) content was observed. These results suggest that the FABP3 and FABP4 may be important genes for meat quality and provides useful information for further studies on their roles in skeletal muscle IMF deposit.