Effect of microRNA-203 on tumor growth in human hypopharyngeal squamous cell carcinoma

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• 作者：Ru Wang ; Jugao Fang ; Hongzhi Ma ; Lin Feng ; Meng Lian…
• 关键词：Hypopharyngeal neoplasm ; Hsa ; mir ; 203 ; Cell proliferation ; Cell cycle
• 刊名：Molecular and Cellular Biochemistry
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：405
• 期：1-2
• 页码：97-104
• 全文大小：2,596 KB
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• 作者单位：Ru Wang (1)
  Jugao Fang (1) (2)
  Hongzhi Ma (1)
  Lin Feng (1)
  Meng Lian (1)
  Fan Yang (1)
  Haizhou Wang (1)
  Qi Wang (1) (2)
  Xiaohong Chen (1) (2)
  
  1. Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730, China
  2. Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Ministry of Education, Beijing Institute of Otorhinolaryngology, Beijing, 100005, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Medical Biochemistry
  Oncology
  Cardiology
  
• 出版者：Springer Netherlands
• ISSN：1573-4919

文摘

MicroRNAs (MiRNAs) have been recognized to regulate cancer initiation and progression in carcinogenesis as either oncogenes or tumor suppressor genes, but their role in hypopharyngeal cancer development is not clearly defined. To determine whether miRNA-203 can promote tumor growth in human hypopharyngeal squamous cell carcinoma, we conducted experiments on the functional study of miRNA-203 and identification of miRNA-203 regulated target genes in hypopharyngeal cancer cells. We found that cell proliferation and cell colony-forming increased more in the miRNA-203 up-regulated cancer cells than in the negative control cancer cells. Up-regulation of miRNA-203 accelerated cell cycle progression in hypopharyngeal cancer cells. TP63 and B3GNT5 mRNAs were identified and validated as targets of miRNA-203. However, transwell assay and wound scratch assay showed that miRNA-203 did not involve in invasion and metastasis in hypopharyngeal cancer cells. According to the results, we conclude that miRNA-203 can promote tumor growth in human hypopharyngeal squamous cell carcinoma. These results provide the convincing evidence for the first time that up-regulation of miRNA-203 contributes to the malignancy of hypopharyngeal squamous cell carcinoma, possibly through down-regulating TP63 and B3GNT5.