Optimization of fermentation conditions for production of xylanase by a newly isolated strain, Penicillium thiersii ZH-19
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- 作者：Fengjie Cui ; Yin Li ; Zhiqiang Liu ; Hui Zhao ; Lifeng Ping ; Liying Ping ; Yinan Yang ; Yaping Xue and Lijiao Yan
- 关键词：Identification ; Penicillium thiersii ZH ; 19 ; Response surface methodology ; Optimization ; Xylanase
- 刊名：World Journal of Microbiology and Biotechnology
- 出版年：2009
- 出版时间：April, 2009
- 年：2009
- 卷：25
- 期：4
- 页码：721-725
- 全文大小：297.8 KB
文摘
The objective of this study was to maximize production of xylanase by a newly isolated strain Penicillium thiersii ZH-19. Response surface methodology was employed to study the effects of significant factors such as pH, temperature, xylan concentration, and cultivation time, on the production of xylanase by Penicillium thiersii ZH-19. The optimal fermentation parameters for enhanced xylanase production were found to be pH 7.72, temperature 24.8¡ãC, xylan 13.2 g l−1 and the fermentation time 125.8 h. The model predicted a xylanase activity of 75.24 U ml−1. Verification of the optimization showed that the maximum xylanase production reached 73.50 U mL−1 in the flask experiments and 80.23 U mL−1 in the scale of 15-L fermenter under the optimal condition.