Application of Plackett–Burman experimental design and Doehlert design to evaluate nutritional requirements for xylanase production by Alternaria mali ND-16

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• 作者：Yin Li ; Zhiqiang Liu ; Fengjie Cui ; Zhisheng Liu and Hui Zhao
• 关键词：Alternaria mali ND ; 16 ; Optimization ; Doehlert design ; Plackett–Burman design ; Xylanase
• 刊名：Applied Microbiology and Biotechnology
• 出版年：2007
• 出版时间：November, 2007
• 年：2007
• 卷：77
• 期：2
• 页码：285-291
• 全文大小：166 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Microbiology
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0614

文摘

The objective of this study was to use statistically based experimental designs for the optimization of xylanase production from Alternaria mali ND-16. Ten components in the medium were screened for nutritional requirements. Three nutritional components, including NH₄Cl, urea, and MgSO₄, were identified to significantly affect the xylanase production by using the Plackett–Burman experimental design. These three major components were subsequently optimized using the Doehlert experimental design. By using response surface methodology and canonical analysis, the optimal concentrations for xylanase production were: NH₄Cl 11.34 g L?1, urea 1.26 g L?1, and MgSO₄ 0.98 g L?1. Under these optimal conditions, the xylanase activity from A. mali ND-16 reached 30.35 U mL?1. Verification of the optimization showed that xylanase production of 31.26 U mL?1 was achieved.