Hepadnavirus detected in bile and liver samples from domestic pigs of commercial abattoirs

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• 作者：Yasmine Rangel Vieira (1)
  Debora Regina Lopes dos Santos (2)
  Moyra Machado Portilho (3)
  Carlos Eduardo Pereira Velloso (1)
  Marcia Arissawa (4)
  Livia Melo Villar (3)
  Marcelo Alves Pinto (1)
  Vanessa Salete de Paula (1)
  
  1. Laborat贸rio de Desenvolvimento Tecnol贸gico em Virologia ; Pavilh茫o H茅lio e Peggy Pereira 鈥?2掳 andar - sala B220 ; Instituto Oswaldo Cruz ; FIOCRUZ ; Av. Brasil ; n掳 4365 ; Manguinhos - RJ ; Cep ; 21045-900 ; Rio de Janeiro ; RJ ; Brasil
  2. Departamento de Mircobiologia Veterin谩ria e Imunologia ; Universidade Federal Rural do Rio de Janeiro ; UFRRJ ; Serop茅dica ; RJ ; Brasil
  3. Laborat贸rio de Hepatites Virais ; Instituto Oswaldo Cruz ; FIOCRUZ ; Rio de Janeiro ; RJ ; Brasil
  4. Laborat贸rio de Tecnologia de Anticorpos Monoclonais ; Bio-Manguinhos ; FIOCRUZ ; Rio de Janeiro ; RJ ; Brasil
  
• 关键词：Hepadnavirus ; Brazil ; Commercial swine ; Bile ; Liver ; Genotyping ; Immunofluorescence
• 刊名：BMC Microbiology
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：14
• 期：1
• 全文大小：2,163 KB
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• 刊物主题：Microbiology; Biological Microscopy; Fungus Genetics; Parasitology; Virology; Life Sciences, general;
• 出版者：BioMed Central
• ISSN：1471-2180

文摘

Background Preliminary studies showed the prevalence of a virus similar to human hepatitis B virus (HBV-like) in swine from farms in China and the molecular evidence of Hepadnavirus infection in domestic pigs herds in Brazil. In this study, we genetically characterize the swine Hepadnavirus strains in swine from slaughterhouses located in certified abattoirs from Rio de Janeiro State, Brazil and evaluate its hepatotropic potential. Results Bile and liver samples from swine were positive for partial genome amplification (ORF S and ORF C), direct sequencing and viral load quantification. Sequencing of the gene encoding the surface antigen allowed classification of Hepadnavirus into genotypes, similar to HBV genotype classification. Indirect immunofluorescence confirmed the presence of HBsAg antigen in liver tissue sections. Conclusions So far our data suggest that commercial swine house an HBV-like virus and this relevant finding should be considered in studies on the origin and viral evolution.