Detection of LINE RT elements in the olive flounder (Paralichthys olivaceus) genome and expression analysis after infection with S. parauberis

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• 作者：Jeong-An Gim ; Gyu-Hwi Nam ; Woo-Jin Kim ; Hee-Eun Lee ; Anshuman Mishra…
• 关键词：Expression ; Long interspersed nuclear elements ; Olive flounder ; Paralichthys olivaceus ; Streptococcus parauberis
• 刊名：Genes & Genomics
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：38
• 期：11
• 页码：1105-1110
• 全文大小：1,057 KB
• 刊物主题：Microbial Genetics and Genomics; Plant Genetics & Genomics; Animal Genetics and Genomics; Human Genetics;
• 出版者：Springer Netherlands
• ISSN：2092-9293
• 卷排序：38

文摘

Long interspersed nuclear elements (LINEs) are widely distributed in the vertebrate genome, and can be either beneficial or detrimental to the host genome. Here we identified three members of LINE RT elements in the olive flounder genome. They showed high amino acid sequence identity (89–99 %), and the sequences of LINE reverse transcriptase (RT) in olive flounder are closely related to those of coral grouper, European seabass, and three-spined stickleback. Real-time reverse transcription-polymerase chain reaction analysis indicated that expression of the OF (Olive flounder)-LINE Chr3-1 RT increased more in spleen than in other tissues after treatment with the pathogen Streptococcus parauberis. These data may form the basis for further studies on the function of retroelements in infected olive flounder.