Production of anti-idiotype antibodies for deoxynivalenol and their evaluation with three immunoassay platforms
详细信息 查看全文
- 作者：C. M. Maragos (1)
- 关键词：Deoxynivalenol ; Antibody ; Immunoassay ; Biosensor ; Fluorescence polarization
- 刊名：Mycotoxin Research
- 出版年：2014
- 出版时间：May 2014
- 年：2014
- 卷：30
- 期：2
- 页码：103-111
- 全文大小：622 KB
- 参考文献：1. Bona CA, Kohler H (1984) Anti-idiotypic antibodies and internal images. In: Venter JC, Fraser CM, Lindstrom J (eds) Monoclonal and anti-idiotypic antibodies: probes for receptor structure and function. Alan R. Liss, New York, pp 141-49
2. Chanh TC, Huot RI, Schick MR, Hewetson JF (1989) Anti-idiotypic antibodies against a monoclonal antibody specific for the trichothecene mycotoxin T-2. Toxicol Appl Pharmacol 100:201-07. doi:10.1016/0041-008X(89)90306-2 CrossRef
3. Chanh TC, Siwak EB, Hewetson JF (1991) Anti-idiotype-based vaccines against biological toxins. Toxicol Appl Pharmacol 108:183-93. doi:10.1016/0041-008X(91)90109-R CrossRef
4. Chanh TC, Kennedy RC, Hewetson JF (1992) Anti-idiotype vaccines in toxicology. Intl J Clin Lab Res 22:28-5. doi:10.1007/BF02591390 CrossRef
5. Chu FS, Huang X, Maragos CM (1995) Production and characterization of anti-idiotype and anti-anti-idiotype antibodies against fumonisin B1. J Agric Food Chem 43:261-67. doi:10.1021/jf00049a046 CrossRef
6. Guan D, Li P, Zhang Q, Zhang W, Cui Y (2011) A competitive immunoassay with a surrogate calibrator curve for aflatoxin M1 in milk. Analytica Chim Acta 703:64-9. doi:10.1016/j.aca.2011.07.011
7. He J, Fan MT, Liang Y, Liu XJ (2010) Application of anti-idiotype antibody in small molecules immunoassay. Chinese J Anal Chem 38:1366-370. doi:10.1016/S1872-2040(09)60068-2 CrossRef
8. Hsu KH, Chu FS (1994) Production and characterization of anti-idiotype and anti-anti-idiotype antibodies from a monoclonal antibody against aflatoxin. J Agric Food Chem 42:2353-359. doi:10.1021/jf00046a052 CrossRef
9. Jerne NK (1974) Towards a network theory of the immune system Ann. Immunol (Inst Pasteur) 125C:373-89
10. Krska R, Welzig E, Boudra H (2007) Analysis of / Fusarium toxins in feed. Anim Feed Sci Technol 137:241-64. doi:10.1016/j.anifeedsci.2007.06.004 CrossRef
11. Krska R, Schubert-Ullrich P, Molinelli A, Sulyok M, MacDonald S, Crews C (2008) Mycotoxin analysis: an update. Food Addit Contam 25:152-63. doi:10.1080/02652030701765723 CrossRef
12. Lippolis V, Pascale M, Visconti A (2006) Optimization of a fluorescence polarization immunoassay for rapid quantification of deoxynivalenol in Durum wheat-based products. J Food Prot 69:2712-719
13. Liu R, He Q, Xu Y, Yu Z (2007) An immunoassay for ochratoxin A without the mycotoxin. Food Control 18:872-77. doi:10.1016/j.foodcont.2006.05.002 CrossRef
14. Maragos CM (2012) Signal amplification using colloidal gold in a biolayer interferometry-based immunosensor for the mycotoxin deoxynivalenol. Food Addit Contam A 29:1108-117. doi:10.1080/19440049.2012.671789 CrossRef
15. Maragos CM, Busman M (2010) Rapid and advanced tools for mycotoxin analysis: a review. Food Addit Contam A 27:1-3. doi:10.1080/19440040903515934 CrossRef
16. Maragos CM, McCormick SP (2000) Monoclonal antibodies for the mycotoxins deoxynivalenol and 3-acetyl-deoxynivalenol. Food Agric Immunol 12:181-92. doi:10.1080/09540100050140722 CrossRef
17. Maragos CM, Plattner RD (2002) Rapid fluorescence polarization immunoassay for the mycotoxin deoxynivalenol in wheat. J Agric Food Chem 50:1827-832. doi:10.1021/jf011487d CrossRef
18. Maragos CM, Li L, Chen D (2012) Production andcharacterization of a single chain variable fragment (scFv) against the mycotoxin deoxynivalenol.Food Agric Immunol 23:51-67
19. Pascale M, Lippolis V, Maragos CM, Visconti A (2008) Recent developments in trichothecene analysis. In: Siantar DP, Trucksess MW, Scott PM, Herman EM (eds) Food Contaminants: ACS Symposium Series 1001. American Chemical Society, Washington DC, pp 192-10 CrossRef
20. Pestka J (2010) Toxicological mechanisms and potential health effects of deoxynivalenol and nivalenol. World Mycotoxin J 3:323-47. doi:10.3920/WMJ2010.1247 CrossRef
21. Rich RL, Myszka DG (2007) Higher-throughput, label-free, real-time molecular interaction analysis. Anal Biochem 361:1-. doi:10.1016/j.ab.2006.10.040 CrossRef
22. Turner PC (2010) Deoxynivalenol and nivalenol occurrence and exposure assessment. World Mycotoxin J 3:315-21. doi:10.3920/WMJ2010.1242 CrossRef
23. Shephard GS, Berthiller F, Dorner J, Krska R, Lombaert GA, Malone B et al (2010).Developments in mycotoxin analysis: an update for 2008-009. World Mycotoxin J3:3-3
24. Turner NW, Subrahmanyam S, Piletsky SA (2009) Analytical methods for determination of mycotoxins: a review. Analytica Chim Acta 632:168-80. doi:10.1016/j.aca.2008.11.010 CrossRef
25. Uner A, Gavalchin J (2006) Idiotypes. Encyclopedia of Life Sciences 1-5. doi:10.1002/9780470015902.a0000912
26. Yu FY, Chu FS (1999) Production and characterization of a monoclonal anti-anti-idiotype antibody against fumonisin B1. J Agric Food Chem 47:4815-820. doi:10.1021/jf990185x CrossRef
27. Yuan Q, Hart PL, Hespenheide BM, Kuhn LA, Pestka JJ, Linz JE (1999) Identification of mimotope peptides which bind to the mycotoxin deoxynivalenol-specific monoclonal antibody. Appl Environ Microbiol 65:3279-286 - 作者单位：C. M. Maragos (1)
1. Bacterial Foodborne Pathogens & Mycology Research Unit, National Center for Agricultural Utilization Research, ARS, USDA, 1815 N. University Street, Peoria, IL, 61604, USA - ISSN：1867-1632
文摘
Immunoassays for deoxynivalenol (DON) that involve binding to DON-specific antibodies have been widely developed. In such assays, the responses of samples are generally compared with calibration curves generated by using DON in competition with labeled reagents such as enzymatic or fluorescent conjugates of the toxin. However, materials that mimic the toxin can also be used, provided that they compete effectively with the labeled reagents for the DON-specific antibodies. Examples include certain types of anti-idiotype antibodies, obtained by the immunization of animals with toxin-specific antibodies. In the present work, anti-idiotype antibodies were developed which mimicked DON in the ability to bind to a DON-specific monoclonal antibody (Mab). Fab fragments of the Mab (Ab1) were used to immunize rabbits. Sera were screened by competitive direct enzyme linked immunosorbent assay (CD-ELISA) for the presence of anti-idiotype antibodies (Ab2). In order to determine the most effective screening format and also the potential efficacy in various forms of biosensors, the sera were further evaluated in biolayer interferometry (BLI) and fluorescence polarization immunoassay (FPIA) formats. All three formats were used to demonstrate the presence of anti-idiotypes capable of binding to the paratope of the DON antibody (subtypes Ab2β or Ab2γ). Such materials have the potential to replace DON as calibrants in immunoassays for this toxin.