Determination of 2,4-dichlorophenoxyacetic acid in cereals by fluorescence polarization immunoassay

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• 作者：A. Yu. Boroduleva ; S. A. Eremin
• 刊名：Journal of Analytical Chemistry
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：71
• 期：9
• 页码：949-954
• 全文大小：226 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Russian Library of Science
  
• 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
• ISSN：1608-3199
• 卷排序：71

文摘

A fluorescence polarization immunoassay (FPIA) procedure is developed for the determination of 2,4-dichlorophenoxyacetic acid in cereal grains using a Sentry-200 portable device. We synthesized tracers, that is, antigens labeled with fluorescein derivatives, based on two fluorescent compounds and two chlorophenoxyacids. The tracer synthesized from 2,4-dichlorophenoxyacetic acid and 4-aminomethylfluorescein is found to be optimal for the FPIA of 2,4-dichlorophenoxyacetic acid. The limit of detection for 2,4-dichlorophenoxyacetic acid in flour was 40 ng/g; the analytical range is from 80 to 1000 ng/g. The percentage of recovery was 85 ± 7% at the relative standard deviation (RSD) 2–10%.Keywordsfluorescence polarization immunoassaypesticide2,4-dichlorophenoxyacetic acidchlorophenoxyacids