Investigating the biological response of human mesenchymal stem cells to titanium surfaces

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• 作者：Matthew J German (1)
  Charles Osei-Bempong (2)
  Callie A Knuth (3)
  David J Deehan (4)
  Rachel A Oldershaw (3) (5)
  
  1. Centre for Oral Health Research ; School of Dental Sciences ; Faculty of Medical Sciences ; Newcastle University ; Newcastle upon Tyne ; NE2 4BW ; UK
  2. Institute of Genetic Medicine ; Faculty of Medical Sciences ; Newcastle University ; International Centre for Life ; Times Square ; Newcastle upon Tyne ; NE1 4EP ; UK
  3. North East England Stem Cell Institute ; Faculty of Medical Sciences ; Newcastle University ; International Centre for Life ; Times Square ; Newcastle upon Tyne ; NE1 4EP ; UK
  4. Department of Orthopaedics ; Freeman Hospital ; Newcastle upon Tyne NHS Hospitals Foundation Trust ; Freeman Road ; High Heaton ; Newcastle upon Tyne ; NE7 7DN ; UK
  5. Department of Musculoskeletal Biology ; Institute of Ageing and Chronic Disease ; Faculty of Health and Life Sciences ; The University of Liverpool ; Leahurst Campus ; Chester High Road ; Neston ; CH64 7TE ; UK
  
• 关键词：Titanium screws ; Tissue engineering ; Mesenchymal stem cells ; Biocompatibility
• 刊名：Journal of Orthopaedic Surgery and Research
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：9
• 期：1
• 全文大小：1,273 KB
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• 刊物主题：Orthopedics; Surgical Orthopedics;
• 出版者：BioMed Central
• ISSN：1749-799X

文摘

Background We have investigated the behaviour of a newly characterised population of haemarthrosis fluid-derived human mesenchymal stem cells (HF-hMSCs) with titanium (Ti) surfaces. Methods HF-hMSCs were seeded onto round cannulated interference (RCI; Smith and Nephew) screws or control Ti discs and cultured under pro-osteogenic conditions. Results Electron microscopy showed the attachment and spreading of HF-hMSCs across both Ti surfaces during the early stages of osteogenic culture; however, cells were exclusively localised to the basal regions within the vertex of the Ti screws. In the later stages of culture, an osteoid matrix was deposited on the Ti surfaces with progressive culture expansion and matrix deposition up the sides and the top of the Ti Screws. Quantification of cellular content revealed a significantly higher number of cells within the Ti screw cultures; however, there was no difference in the cellular health. Conversely, alizarin red staining used as both a qualitative and quantitative measure of matrix calcification was significantly increased in Ti disc cultures compared to those of Ti screws. Conclusions Our results suggest that the gross topography of the metal implant is able to create microenvironment niches that have an influence on cellular behaviour. These results have implications for the design of advanced tissue engineering strategies that seek to use cellular material to enhance biological remodelling and healing following tissue reconstruction.