Action modes of recombinant endocellulase, EGA, and its domains on cotton fabrics

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• 作者：Meilan Yu ; Yaqin Qiu ; Wei Chen ; Fukun Zhao ; Jianzhong Shao
• 关键词：Carbohydrate ; binding module ; Catalytic domain ; Cotton fiber ; Degree of polymerization ; Endocellulase ; Endoglucanase ; Fabric strength
• 刊名：Biotechnology Letters
• 出版年：2015
• 出版时间：August 2015
• 年：2015
• 卷：37
• 期：8
• 页码：1615-1622
• 全文大小：1,041 KB
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• 作者单位：Meilan Yu (1) (2)
  Yaqin Qiu (1)
  Wei Chen (1)
  Fukun Zhao (1)
  Jianzhong Shao (2)
  
  1. College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, China
  2. Engineering Research Center for Eco-Dyeing & Finishing of Textiles, Ministry of Education, Zhejiang Sci-Tech University, Hangzhou, 310018, Zhejiang, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Biotechnology
  Applied Microbiology
  Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-6776

文摘

Objectives The action modes of an endocellulase, EGA, and its domains (CD9 and CBM3) during enzymatic treatment of cotton fabrics were investigated. Results EGA, CD9 and CBM3 had the binding capacity to cellulose substrates, of which the filter paper was the substrate with the strongest binding capacity. Analyses of scanning electronic microscopy indicated that EGA and its catalytic domain CD9 etched the surface of cotton fabrics and broke the fibers of long chains. On the other hand, the binding domain CBM3 only resulted in swelling of cotton fibers. Both EGA and its catalytic domain CD9 had minimal effect on the weight loss of cotton fabrics, whereas the effect of EGA and CD9 on the degree of polymerization and breaking strength was significant. After 12?h enzymatic action, the values of weight loss ratio for EGA and CD9 were 2.07 and 2.21?%, respectively, meanwhile the reductions in fabric strength were 27.04?% for EGA and 17.23?% for CD9. Conclusions In contrast to the action of EGA and CD9, CBM3 showed no significant changes in terms of the weight loss ratio, degree of polymerization, and fabric strength.