Engineering of Bacillus subtilis for the Production of 2,3-Butanediol from Sugarcane Molasses

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• 作者：Apoorva Nandkumar Deshmukh…
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：179
• 期：2
• 页码：321-331
• 全文大小：313 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Biochemistry
  
• 出版者：Humana Press Inc.
• ISSN：1559-0291
• 卷排序：179

文摘

2,3-butanediol is known to be a platform chemical with several potential industrial applications. Sustainable industrial scale production can be attained by using a sugarcane molasses based fermentation process using Bacillus subtilis. However, the accumulation of acetoin needs to be reduced to improve process efficiency. In this work, B. subtilis was genetically modified in order to increase the yield of 2,3-butanediol. Metabolic engineering strategies such as cofactor engineering and overexpression of the key enzyme butanediol dehydrogenase were attempted. Both the strategies individually led to a statistically significant increase in the 2,3-butanediol yields for sugarcane molasses based fermentation. Cofactor engineering led to a 26 % increase in 2,3-butanediol yield and overexpression of bdhA led to a 11 % increase. However, the combination of the two strategies did not lead to a synergistic increase in 2,3-butanediol yield.Keywords2,3-butanediolSugarcane molassesBacillus subtilisCofactor engineeringEnzyme overexpression