Application of quantitative 1H-NMR method to determination of gentiopicroside in Gentianae radix and Gentianae scabrae radix
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- 作者：Rie Tanaka (1)
Yuko Hasebe (1)
Akito Nagatsu (1) - 关键词：Gentiopicroside ; Quantitative 1H ; NMR ; Gentianae radix ; Gentianae scabrae radix ; Reagent purity
- 刊名：Journal of Natural Medicines
- 出版年：2014
- 出版时间：July 2014
- 年：2014
- 卷：68
- 期：3
- 页码：630-635
- 全文大小：
- 参考文献：1. Ministry of Health and Welfare (2011) The Japanese Pharmacopeia, 16th edn. Japan, pp 1644
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Yuko Hasebe (1)
Akito Nagatsu (1)
1. Laboratory of Pharmacognosy, College of Pharmacy, Kinjo Gakuin University, 2-1723 Omori, Moriyama-ku, Nagoya, 463-8521, Japan - ISSN：1861-0293
文摘
A quantitative 1H-NMR method (qHNMR) was used to measure gentiopicroside content in Gentianae radix and Gentianae scabrae radix. Gentiopicroside is a major component of Gentianae radix and Gentianae scabrae radix. The purity of gentiopicroside was calculated from the ratio of the intensity of the H-3 signal at δ 7.44?ppm or the H-8 signal at δ 5.78?ppm in methanol-d 4 of gentiopicroside to that of a hexamethyldisilane (HMD) signal at 0?ppm. The concentration of HMD was corrected with SI traceability by using potassium hydrogen phthalate of certified reference material (CRM) grade. As a result, the gentiopicroside content in two lots of Gentianae radix as determined by qHNMR was found to be 1.76 and 2.17?%, respectively. The gentiopicroside content in two lots of Gentianae scabrae radix was 2.73 and 3.99?%, respectively. We demonstrated that this method is useful for the quantitative analysis of crude drugs.