Characterization of a new aryl-alcohol oxidase secreted by the phytopathogenic fungus Ustilago maydis

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• 作者：Marie Couturier ; Yann Mathieu ; Ai Li…
• 关键词：Aryl ; alcohol oxidase ; Ustilago maydis ; CAZy ; AA3 ; Lignin ; Biorefinery
• 刊名：Applied Microbiology and Biotechnology
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：100
• 期：2
• 页码：697-706
• 全文大小：1,247 KB
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• 作者单位：Marie Couturier (1) (2)
  Yann Mathieu (1) (2)
  Ai Li (3)
  David Navarro (1) (2)
  Elodie Drula (1) (2) (4) (5)
  Mireille Haon (1) (2)
  Sacha Grisel (1) (2)
  Roland Ludwig (3)
  Jean-Guy Berrin (1) (2) (6)
  
  1. INRA, UMR1163 BBF, Polytech’Marseille, F-13288, Marseille, France
  2. Marseille Université, UMR1163 BBF, Polytech’Marseille, F-13288, Marseille, France
  3. Department of Food Science and Technology, University of Natural Resources and Life Sciences, 1190, Vienna, Austria
  4. CNRS, UMR7257 Architecture et Fonction des Macromolécules Biologiques, F-13288, Marseille, France
  5. INRA, USC1408 Architecture et Fonction des Macromolécules Biologiques, F-13288, Marseille, France
  6. INRA, Laboratoire de Biodiversité et Biotechnologie Fongique, Marseille, France
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Microbiology
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0614

文摘

The discovery of novel fungal lignocellulolytic enzymes is essential to improve the breakdown of plant biomass for the production of second-generation biofuels or biobased materials in green biorefineries. We previously reported that Ustilago maydis grown on maize secreted a diverse set of lignocellulose-acting enzymes including hemicellulases and putative oxidoreductases. One of the most abundant proteins of the secretome was a putative glucose-methanol-choline (GMC) oxidoreductase. The phylogenetic prediction of its function was hampered by the few characterized members within its clade. Therefore, we cloned the gene and produced the recombinant protein to high yield in Pichia pastoris. Functional screening using a library of substrates revealed that this enzyme was able to oxidize several aromatic alcohols. Of the tested aryl-alcohols, the highest oxidation rate was obtained with 4-anisyl alcohol. Oxygen, 1,4-benzoquinone, and 2,6-dichloroindophenol can serve as electron acceptors. This GMC oxidoreductase displays the characteristics of an aryl-alcohol oxidase (E.C.1.1.3.7), which is suggested to act on the lignin fraction in biomass. Keywords Aryl-alcohol oxidase Ustilago maydis CAZy AA3 Lignin Biorefinery