HIV signaling through CD4 and CCR5 activates Rho family GTPases that are required for optimal infection of primary CD4+ T cells
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- 作者：Mark B. Lucera ; Zach Fleissner ; Caroline O. Tabler ; Daniela M. Schlatzer…
- 关键词：HIV ; 1 ; Small molecule screen ; Rho GTPases ; ROCK ; Cdc42 ; Viral infection
- 刊名：Retrovirology
- 出版年：2017
- 出版时间：December 2017
- 年：2017
- 卷：14
- 期：1
- 全文大小：1106KB
- 刊物主题：Virology; Infectious Diseases; Cancer Research; Vaccine; Antibodies; Protein Structure;
- 出版者：BioMed Central
- ISSN：1742-4690
- 卷排序：14
文摘
BackgroundHIV-1 hijacks host cell machinery to ensure successful replication, including cytoskeletal components for intracellular trafficking, nucleoproteins for pre-integration complex import, and the ESCRT pathway for assembly and budding. It is widely appreciated that cellular post-translational modifications (PTMs) regulate protein activity within cells; however, little is known about how PTMs influence HIV replication. Previously, we reported that blocking deacetylation of tubulin using histone deacetylase inhibitors promoted the kinetics and efficiency of early post-entry viral events. To uncover additional PTMs that modulate entry and early post-entry stages in HIV infection, we employed a flow cytometric approach to assess a panel of small molecule inhibitors on viral fusion and LTR promoter-driven gene expression.