Radical scavenging activity of chitooligosaccharide with different molecular weights

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• 作者：Yumin Yang ; Rengeng Shu ; Jian Shao ; Guangfei Xu and Xingxing Gu
• 关键词：Antioxidants ; Chitooligosaccharide ; Chitosan ; Reactive oxygen species ; Different molecular weight
• 刊名：European Food Research and Technology
• 出版年：2006
• 出版时间：January 2006
• 年：2006
• 卷：222
• 期：1-2
• 页码：36-40
• 全文大小：300 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Food Science
  Analytical Chemistry
  Biotechnology
  Agriculture
  Forestry
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1438-2385

文摘

Two chitooligosaccharides, Ch1100 (average molecule weight: 1,100; average degree of polymer: 7) and Ch500 (average molecule weight: 500; average degree of polymer: 3), were obtained by partial hydrolysis of chitosan. Their antioxidant activities were investigated with scavenging activity of chitooligosaccharide against superoxide radical using phenazin methosulphate (PMS)–β-nicotinamide adenine dinucleotide (NADH)–nitroblue tetrazolium (NBT) system, scavenging activity of chitooligosaccharide against carcinogen-induced active oxygen species and scavenging activity of chitooligosaccharide against hydrogen peroxide released from polymorphonuclear leukocytes stimulated by phorbol-12-myristate-13-acetate (PMA). By the aforementioned three measurements, the radical scavenging activity (Inhibition%) of Ch1100 was determined to 54.8, 35.3 and 38.9%, respectively, at the concentration of 100 μmol/L, while that of Ch500 was 40.1, 13.9 and 18.2%, respectively, at the same concentration. These results clearly suggest that Ch1100 is a potent radical scavenger, which has the high radical scavenging activity.