MCM7 Expression Is Altered in Rat After Spinal Cord Injury
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- 作者：Jiajia Chen (1)
Zhiming Cui (1)
Weidong Li (1)
Aiguo Shen (2)
Guanhua Xu (1)
Guofeng Bao (1)
Yuyu Sun (1)
Lingling Wang (1)
Jianbo Fan (1)
Jinlong Zhang (1)
Longfei Yang (1)
Zhiming Cui (1) - 关键词：Spinal cord injury ; MCM7 ; Neuronal apoptosis ; Proliferation ; Rat
- 刊名：Journal of Molecular Neuroscience
- 出版年：2013
- 出版时间：September 2013
- 年：2013
- 卷：51
- 期：1
- 页码：82-91
- 全文大小：842KB
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Zhiming Cui (1)
Weidong Li (1)
Aiguo Shen (2)
Guanhua Xu (1)
Guofeng Bao (1)
Yuyu Sun (1)
Lingling Wang (1)
Jianbo Fan (1)
Jinlong Zhang (1)
Longfei Yang (1)
Zhiming Cui (1)
1. Department of Spine Surgery, The Second Affiliated Hospital of Nantong University, Nantong University, 226001, Nantong, Jiangsu, People鈥檚 Republic of China
2. Department of Immunology, Medical College, Nantong University, 226001, Nantong, Jiangsu, People鈥檚 Republic of China
文摘
Minichromosome maintenance protein 7 (MCM7), a member of the minichromosome maintenance protein family, is essential for eukaryotic DNA replication initiation and the early stage of the elongation process. MCM7 participates in the cell cycle control of genome duplication. While it is ubiquitously expressed in all tissues, the biological function of MCM7 in the central nervous system is still with limited acquaintance. In the present study, we performed a spinal cord injury (SCI) model in adult rats. Western blotting indicated a marked alteration of MCM7 after SCI. Immunohistochemistry analysis revealed a wide distribution of MCM7 in the spinal cord. Double immunofluorescence staining showed that MCM7 immunoreactivity was increased predominantly in neurons, astrocytes, and microglia after SCI. We also examined the expression profiles of active caspase-3, proliferating cell nuclear antigen (PCNA), and Ki67, whose changes were correlated with the expression profiles of MCM7. Moreover, colocalization of MCM7/active caspase-3 was detected in neuronal nuclei (NeuN), and colocalization of MCM7/PCNA was detected in NeuN, glial fibrillary acidic protein, and CD11b, respectively. Our results suggest that MCM7 might be implicated in the apoptosis of neuron and proliferation of astrocytes and microglia after SCI.