Monitoring enzyme reaction and screening enzyme inhibitor based on MALDI-TOF-MS platform with a matrix of oxidized carbon nanotubes
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- 作者：Ligang Hu (1) (2)
Guibin Jiang (1)
Songyun Xu (2)
Chensong Pan (2)
Hanfa Zou (2) - 刊名：Journal of The American Society for Mass Spectrometry
- 出版年：2006
- 出版时间：November 2006
- 年：2006
- 卷：17
- 期：11
- 页码：1616-1619
- 全文大小：210KB
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11. Hu, L.; Xu, S.; Pan, C.; Yuan, C.; Zou, H.; Jiang, G. Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry with a Matrix of Carbon Nanotubes for the Analysis of Low-mass Compounds in Environmental Samples. / Environ. Sci. Technol 2005, / 39, 8442鈥?447. CrossRef - 作者单位：Ligang Hu (1) (2)
Guibin Jiang (1)
Songyun Xu (2)
Chensong Pan (2)
Hanfa Zou (2)
1. State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuangqing Road, 100085, Beijing, China
2. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China
文摘
A matrix assisted laser desorption/ionization time-of-flight mass spectrometry platform for quantitatively monitoring enzyme activity and screening enzyme inhibitors has been demonstrated. The described method employs a new matrix of oxidized carbon nanotubes. Compared with the traditional fluorescence approach, this label-free method has the advantage of directly identifying the substrates and products in enzymatic reactions. Moreover, the method could be conveniently carried out with any commercial mass spectrometer without modification. We quantitatively monitored the acetylcholinesterase activity and screened acetylcholinesterase inhibitors with a detection rate of about 3.3 s per sample.