Expression and function of NET-1 in human skin squamous cell carcinoma

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• 作者：Jie zhang (1)
  Jianli Wang (1) (2)
  Li Chen (2)
  Guilan Wang (2)
  Jing Qin (2)
  Yuyin Xu (1)
  Xingyu Li (2)
  
• 关键词：Skin squamous cell carcinoma (SSCC) ; NET ; 1 (TSPAN1 ; TSPAN1/C4-) ; RNA interference (RNAi)
• 刊名：Archives of Dermatological Research
• 出版年：2014
• 出版时间：May 2014
• 年：2014
• 卷：306
• 期：4
• 页码：385-397
• 全文大小：
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• 作者单位：Jie zhang (1)
  Jianli Wang (1) (2)
  Li Chen (2)
  Guilan Wang (2)
  Jing Qin (2)
  Yuyin Xu (1)
  Xingyu Li (2)
  
  1. Dermatology Department, Affiliated Hospital of Nantong University, Nantong, 226001, People’s Republic of China
  2. Department of Pathology, School of Medicine, Nantong University, Nantong, 226001, People’s Republic of China
  
• ISSN：1432-069X

文摘

To evaluate the clinicopathological significance of NET-1 in human skin squamous cell carcinoma (SSCC). The expression of NET-1 and Ki67 protein was detected using immunostaining from 60 SSCC cases, 50 SIN samples and ten normal skin tissues. The vectors expressing NET-1, siRNA NET-1 and shRNA NET-1 were constructed, as well as negative controls (target-off). In transfected A431 cells, the expression of NET-1 was detected by qRT-PCR, Western blot and immunofluorescence staining; the proliferation and migration of cells was evaluated by MTT, flow cytometry, wound healing and transwell chamber assays. The stable cell lines transfected with shRNANET-1 was inoculated in nude mice for in vivo study. (1) The levels of NET-1 were significantly higher in SSCC (96.67?%) and SIN III (93.75?%) than that in SIN I and II (41.18?%), (P?<?0.05). NET-1 expression was significantly enhanced in spindle-cell SSCC (75?%) versus other histological types (P?<?0.05). (2) The expression of NET-1 in A431 cells transfected with siRNANET-1 or shRNANET-1 was significantly decreased; the proliferation and migration of these cells were obviously inhibited as compared to controls (P?<?0.05). (3) The growth of subcutaneous tumors was significantly inhibited associated with reduction in the expression of NET-1 vs. the negative control or untreated group (P?<?0.05). The overexpression of NET-1 in tumor cells may be closely related to the malignant phenotype of SSCC. NET-1 RNAi used in this study can specifically and effectively downregulate NET-1 gene expression; thus SSCC proliferation, invasion and tumor growth were attenuated. NET-1 might be one of the potential targets for SSCC therapy.