Using patient-collected clinical samples and sera to detect and quantify the severe acute respiratory syndrome coronavirus (SARS-CoV)

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• 作者：Zhongping He (1) (2)
  Hui Zhuang (3)
  Chunhui Zhao (2)
  Qingming Dong (1)
  Guoai Peng (1)
  Dominic E Dwyer (4)
  
• 刊名：Virology Journal
• 出版年：2007
• 出版时间：December 2007
• 年：2007
• 卷：4
• 期：1
• 全文大小：172KB
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• 作者单位：Zhongping He (1) (2)
  Hui Zhuang (3)
  Chunhui Zhao (2)
  Qingming Dong (1)
  Guoai Peng (1)
  Dominic E Dwyer (4)
  
  1. Beijing Ditan Hospital, Beijing, 100011, People's Republic of China
  2. Capital University of Medical Sciences Affiliated Beijing YouAn Hospital, Beijing, 100054, People's Republic of China
  3. Department of Microbiology, Peking University Health Science Center, Beijing, 100083, People's Republic of China
  4. Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research, Westmead Hospital, Westmead, NSW, 2145, Australia
  
• ISSN：1743-422X

文摘

Background Severe acute respiratory syndrome (SARS) caused a large outbreak of pneumonia in Beijing, China, in 2003. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect and quantify SARS-CoV in 934 sera and self-collected throat washes and fecal samples from 271 patients with laboratory-confirmed SARS managed at a single institution. Results SARS-CoV detection rates in sera were highest in the first 9 days of illness, whereas detection was highest in throat washes 5-4 days after onset of symptoms. The highest SARS-CoV RT-PCR rates (70.4-6.3%) and viral loads (log10 4.5-.1) were seen in fecal samples collected 2- weeks after the onset of clinical illness. Fecal samples were frequently SARS-CoV RT-PCR positive beyond 40 days, and occasional sera still had SARS-CoV detected after 3 weeks of illness. Conclusion In the context of an extensive outbreak with major pressure on hospital resources, patient self-collected samples are an alternative to nasopharyngeal aspirates for laboratory confirmation of SARS-CoV infection.