Overexpression of Wnt7α protein predicts poor survival in patients with colorectal carcinoma

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• 作者：Yichong Wang ; Jiufeng Wei ; Shujun Zhang ; Guodong Li ; Tao Zhang ; Xin Yu…
• 关键词：Colorectal cancer ; Wnt7α ; Prognosis ; Immunohistochemistry
• 刊名：Tumor Biology
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：36
• 期：11
• 页码：8781-8787
• 全文大小：801 KB
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• 作者单位：Yichong Wang (1)
  Jiufeng Wei (1)
  Shujun Zhang (2)
  Guodong Li (1)
  Tao Zhang (3)
  Xin Yu (4)
  Hongsheng Chen (1)
  Ming Liu (1)
  
  1. Department of General Surgery, The Fourth Affiliated Hospital, Harbin Medical University, Harbin, 150081, China
  2. Department of Pathology, The Fourth Affiliated Hospital, Harbin Medical University, Harbin, 150081, China
  3. Department of Orthopedics, The Fourth Affiliated Hospital, Harbin Medical University, Harbin, 150081, China
  4. Department of Gastroenterology, The Fourth Affiliated Hospital, Harbin Medical University, Harbin, 150081, China
  
• 刊物主题：Cancer Research;
• 出版者：Springer Netherlands
• ISSN：1423-0380

文摘

Wnt7α (wingless-type MMTV integration site family, member 7A) is a secreted glycoprotein that plays a critical role in tumorigenesis and development by controlling cell proliferation and differentiation. Whether Wnt7α has the properties of an oncogene or not is an interesting issue because of its diverse expression in different tumors. In the present study, Wnt7α protein expression was evaluated through immunohistochemistry and Western blot analysis. Univariate and multivariate analyses were applied to explore the associations between Wnt7α staining score and various clinical parameters, including overall survival (OS) and disease-free survival (DFS), and a total of 212 patients with colorectal cancer (CRC) were surveyed. Wnt7α was strongly expressed in most CRC tissues but weakly expressed in adjacent normal mucosa, colorectal adenomas, and colonic polyps. High levels of Wnt7α expression were strongly associated with tumor size (P--.006), lymph node involvement (P-lt;-.001), and the international tumor–node–metastasis (TNM) stage (P--.005). Patients with strong Wnt7α expression showed significantly poorer OS and DFS than patients with weak Wnt7α expression (P-lt;-.0001, both). Multivariate Cox analysis confirmed that Wnt7α protein expression and TNM stage are independent factors of adverse OS and DFS in CRC patients. Taken together, our results present evidence that Wnt7α overexpression is associated with an unfavorable prognosis and that positive Wnt7α, in addition to TNM stage, may be an independent prognosis factor influencing OS and DFS prediction in CRC patients. Keywords Colorectal cancer Wnt7α Prognosis Immunohistochemistry