Rice WRKY4 acts as a transcriptional activator mediating defense responses toward Rhizoctonia solani, the causing agent of rice sheath blight

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• 作者：Haihua Wang ; Jiao Meng ; Xixu Peng ; Xinke Tang ; Pinglan Zhou…
• 关键词：Disease resistance ; Rhizoctonia solani ; Transcription factor ; WRKY protein ; Oryza sativa
• 刊名：Plant Molecular Biology
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：89
• 期：1-2
• 页码：157-171
• 全文大小：4,029 KB
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• 作者单位：Haihua Wang (1) (2) (3)
  Jiao Meng (1)
  Xixu Peng (1) (3)
  Xinke Tang (1) (2)
  Pinglan Zhou (1) (2)
  Jianhua Xiang (1)
  Xiaobo Deng (1)
  
  1. School of Life Science, Hunan University of Science and Technology, Taoyuan Rd., Xiangtan, 411201, Hunan, China
  2. Key Laboratory of Integrated Management of the Pests and Diseases on Horticultural Crops in Hunan Province, Xiangtan, 411201, Hunan, China
  3. Key Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-Polluted Soils, College of Hunan Province, Xiangtan, 411201, Hunan, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Biochemistry
  Plant Pathology
  
• 出版者：Springer Netherlands
• ISSN：1573-5028

文摘

WRKY transcription factors have been implicated in the regulation of transcriptional reprogramming associated with various plant processes but most notably with plant defense responses to pathogens. Here we demonstrate that expression of rice WRKY4 gene (OsWRKY4) was rapidly and strongly induced upon infection of Rhizoctonia solani, the causing agent of rice sheath blight, and exogenous jasmonic acid (JA) and ethylene (ET). OsWRKY4 is localized to the nucleus of plant cells and possesses transcriptional activation ability. Modulation of OsWRKY4 transcript levels by constitutive overexpression increases resistance to the necrotrophic sheath blight fungus, concomitant with elevated expression of JA- and ET-responsive pathogenesis-related (PR) genes such as PR1a, PR1b, PR5 and PR10/PBZ1. Suppression by RNA interference (RNAi), on the other hand, compromises resistance to the fungal pathogen. Yeast one-hybrid assay and transient expression in tobacco cells reveal that OsWRKY4 specifically binds to the promoter regions of PR1b and PR5 which contain W-box (TTGAC[C/T]), or W-box like (TGAC[C/T]) cis-elements. In conclusion, we propose that OsWRKY4 functions as an important positive regulator that is implicated in the defense responses to rice sheath blight via JA/ET-dependent signal pathway. Keywords Disease resistance Rhizoctonia solani Transcription factor WRKY protein Oryza sativa