Identification, characterization of selenoprotein W and its mRNA expression patterns in response to somatostatin 14, cysteamine hydrochloride, 17β-estradiol and a binary mixture of 17β-estradiol and cysteamine hydrochloride in topmouth culter (Erythroculter ilishaeformis)
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- 作者：Haiyan Dong ; Wenbo Chen ; Chao Sun ; Jianwei Sun…
- 关键词：Selenoprotein W ; Somatostatin 14 ; Cysteamine hydrochloride ; 17β ; Estradiol ; Injection
- 刊名：Fish Physiology and Biochemistry
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：43
- 期：1
- 页码：115-126
- 全文大小：
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Freshwater & Marine Ecology; Animal Physiology; Animal Anatomy / Morphology / Histology; Animal Biochemistry; Zoology;
- 出版者：Springer Netherlands
- ISSN：1573-5168
- 卷排序：43
文摘
In this study, a selenoprotein W cDNA was cloned from topmouth culter (Erythroculter ilishaeformis), and it was designated as EISelW. The EISelW open reading frame was composed of 261 base pairs (bp), encoding 86-amino-acid protein. The 5′ untranslated region (UTR) consisted of 104 bp, and the 3′-UTR was composed of 365 bp. A selenocysteine insertion sequence (SECIS) element was found in the 3′-UTR of EISelW mRNA. The SECIS element was classified as form II because of a small additional apical loop presented in SECIS element of EISelW mRNA. Bioinformatic approaches showed that the secondary structure of EISelW was a β1-α1-β2-β3-β4-α2 pattern from amino-terminal to carboxy-terminal. Real-time PCR analysis of EISelW mRNAs expression in 17 tissues showed that the EISelW mRNA was predominantly expressed in liver, ovary, pituitary, various regions of the brain, spinal cord and head kidney. Study of intraperitoneal injection showed that the levels of EISelW mRNA in brain, liver, ovary and spleen were regulated by somatostatin 14 (SS14), 17β-estradiol (E2), cysteamine hydrochloride (CSH) and a binary mixture of E2 and CSH, dependent on the dosage. These results suggest that E2, SS14 and CSH status may affect tissues of selenium metabolism by regulating the expression of SelW mRNA, as SelW plays a central role in selenium metabolism.