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Construction of self-cloning, indigenous wine strains of Saccharomyces cerevisiae with enhanced glycerol and glutathione production
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  • 作者:Rui-Ying Hao (12)
    Yan-Lin Liu (1) lylsun@yahoo.com.cn
    Zhao-Yue Wang (2) wangzhaoyue@126.com
    Bo-run Zhang (2)
  • 关键词:Glutathione – ; Glycerol – ; Indigenous yeast – ; Saccharomyces cerevisiae – ; Self ; cloning – ; Wine
  • 刊名:Biotechnology Letters
  • 出版年:2012
  • 出版时间:September 2012
  • 年:2012
  • 卷:34
  • 期:9
  • 页码:1711-1717
  • 全文大小:363.6 KB
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  • 作者单位:1. College of Enology, Northwest A&F University, Yangling, Shaanxi 712100, People鈥檚 Republic of China2. The Laboratory of Molecular Genetics and Breeding of Yeast, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101 People鈥檚 Republic of China
  • ISSN:1573-6776
文摘
To improve wine taste and flavor stability, a novel indigenous strain of Saccharomyces cerevisiae with enhanced glycerol and glutathione (GSH) production for winemaking was constructed. ALD6 encoding an aldehyde dehydrogenases of the indigenous yeast was replaced by a GPD1 and CUP1 gene cassette, which are responsible for NAD-dependent glycerol-3-phosphatase dehydrogenase and copper resistance, respectively. Furthermore, the α-acetohydroxyacid synthase gene ILV2 of the indigenous yeast was disrupted by integration of the GSH1 gene which encodes γ-glutamylcysteine synthetase and the CUP1 gene cassette. The fermentation capacity of the recombinant was similar to that of the wild-type strain, with an increase of 21 and 19 % in glycerol and GSH production. No heterologous DNA was harbored in the recombinant in this study.

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