Overexpression of the Monodehydroascorbate Reductase Gene from Non-heading Chinese Cabbage Reduces Ascorbate Level and Growth in Transgenic Tobacco

详细信息    查看全文

• 作者：Jun Ren ; Weike Duan ; Zhongwen Chen ; Shuo Zhang…
• 关键词：Transgenic tobacco ; BcMDHAR ; Ascorbic acid ; Monodehydroascorbate reductase ; Non ; heading Chinese cabbage ; Tobacco
• 刊名：Plant Molecular Biology Reporter
• 出版年：2015
• 出版时间：August 2015
• 年：2015
• 卷：33
• 期：4
• 页码：881-892
• 全文大小：2,438 KB
• 参考文献：Alhagdow M, Mounet F, Gilbert L, Nunes-Nesi A, Garcia V, Just D, Petit J, Beauvoit B, Fernie AR, Rothan C, Baldet P (2007) Silencing of the mitochondrial ascorbate synthesizing enzyme l -galactono-1,4-lactone dehydrogenase affects plant and fruit development in tomato. Plant Physiol 145:1408鈥?422PubMed Central PubMed View Article
  Badejo AA, Tanaka N, Esaka M (2008) Analysis of GDP-d -mannose pyrophosphorylase gene promoter from acerola (Malpighiaglabra) and increase in ascorbate content of transgenic tobacco expressing the acerola gene. Plant Cell Physiol 49:126鈥?32PubMed View Article
  Badejo AA, Eltelib HA, Fukunaga K, Fujikawa Y, Esaka M (2009) Increase in ascorbate content of transgenic tobacco plants overexpressing the acerola (Malpighiaglabra) phosphomannomutase gene. Plant Cell Physiol 50:423鈥?28PubMed View Article
  Bulley SM, Rassam M, Hoser D, Otto W, Sch眉nemann N, Wright M, MacRae E, Gleave A, Laing W (2009) Gene expression studies in kiwifruit and gene over-expression in Arabidopsis indicates that GDP-l -galactoseguanyl transferase is a major control point of vitamin C biosynthesis. J Exp Bot 60:765鈥?78PubMed Central PubMed View Article
  Chen Z, Gallie DR (2006) Dehydroascorbate reductase affects leaf growth, development and function. Plant Physiol 142:775鈥?87PubMed Central PubMed View Article
  Chen Z, Young TE, Ling J, Chang SC, Gallie DR (2003) Increasing vitamin C content of plants through enhanced ascorbate recycling. Proc Natl Acad Sci USA 100:3525鈥?530PubMed Central PubMed View Article
  Conklin PL, Williams EH, Last RL (1996) Environmental stress sensitivity of an ascorbic acid-deficient Arabidopsis mutant. Proc Natl Acad Sci USA 93:9970鈥?974PubMed Central PubMed View Article
  Conklin PL, Norris SR, Wheeler GL, Williams EH, Smirnoff N, Last RL (1999) Genetic evidence for the role of GDP-mannose in plant ascorbicacid (vitamin C) biosynthesis. Proc Natl Acad Sci USA 96:4198鈥?203PubMed Central PubMed View Article
  Cruz-Rus E, Amaya I, Valpuesta V (2012) The challenge of increasing vitamin C content in plant foods. Biotechnol J 7:1110鈥?121PubMed View Article
  Davey MW, Montagu MV, Inz茅 D, Sanmartin M, Kanellis A, Smirnoff N, Benzie IJJ, Strain JJ, Favell D, Fletcher J (2000) Plant l -ascorbic acid: chemistry, function, metabolism, bioavailability and effects of processing. J Sci Food Agric 80:825鈥?60View Article
  Dowdle J, Ishikawa T, Gatzek S, Rolinski S, Smirnoff N (2007) Two genes in Arabidopsis thaliana encoding GDP-l -galactose phosphorylase are required for ascorbate biosynthesis and seedling viability. Plant J 52:673鈥?89PubMed View Article
  Eltayeb AE, Kawano N, Badawi GH, Kaminaka H, Sanekata T, Shibahara T, Inanaga S, Tanaka K (2007) Overexpression of monodehydroascorbate reductase in transgenic tobacco confers enhanced tolerance to ozone, salt and polyethylene glycol stresses. Planta 225:1255鈥?264PubMed View Article
  Eltelib HA, Fujikawa Y, Esaka M (2012) Overexpression of the acerola (Malpighia glabra) monodehydroascorbate reductase gene in transgenic tobacco plants results in increased ascorbate levels and enhanced tolerance to salt stress. S Afr J Bot 78:295鈥?01View Article
  Foyer CH, Noctor G (2011) Ascorbate and glutathione: the heart of the redox hub. Plant Physiol 155:2鈥?8PubMed Central PubMed View Article
  Gallie DR (2013) The role of L-ascorbic acid recycling in responding to environmental stress and in promoting plant growth. J Exp Bot 64:433鈥?43PubMed View Article
  Gest N, Garchery C, Gautier H, Jim茅nez A, Stevens R (2013) Light-dependent regulation of ascorbate in tomato by a monodehydroascorbate reductase localized in peroxisomes and the cytosol. Plant Biotechnol J 11:344鈥?54PubMed View Article
  Grantz AA, Brummell DA, Bennett AB (1995) Ascorbate free radical reductase mRNA levels are induced by wounding. Plant Physiol 108:411鈥?18PubMed Central PubMed View Article
  Haroldsen VM, Chi-Ham CL, Kulkarni S, Lorence A, Bennett AB (2011) Constitutively expressed DHAR and MDHAR influence fruit, but not foliar ascorbate levels in tomato. Plant Physiol Biochem 49:1244鈥?249PubMed Central PubMed View Article
  Horemans N, Foyer CH, Asard H (2000) Transport and action of ascorbateat the plant plasma membrane. Trends Plant Sci 5:263鈥?67PubMed View Article
  Horsch RB, Fry JE, Hoffmann NL, Eichholta D, Rpgers SG, Fraley RT (1985) A simple and general method for transferring genes into plants. Science 227:1229鈥?231View Article
  Horton P, Park KJ, Obayashi T, Fujita N, Harada H, Adams-Collier CJ, Nakai K (2007) WoLF PSORT: protein localization predictor. Nucleic Acids Res 35:W585鈥揥587PubMed Central PubMed View Article
  Ishikawa T, Dowdle J, Smirnoff N (2006) Progress in manipulating ascorbic acid biosynthesis and accumulation in plants. Physiol Plant 126:343鈥?55View Article
  Kampfenkel K, Vanmontagu M, Inze D (1995) Extraction and determination of ascorbate and dehydroascorbate from plant tissue. Anal Biochem 225:165鈥?67PubMed View Article
  Kavitha K, George S, Venkataraman G, Parida A (2010) A salt-inducible chloroplastic monodehydroascorbate reductase from halophyte Avicennia marina confers salt stress tolerance on transgenic plants. Biochimie 92:1321鈥?329PubMed View Article
  Laing WA, Wright MA, Cooney J, Bulley SM (2007) The missing step of the l -galactose pathway of ascorbate biosynthesis in plants, an L-galactose guanyltransferase, increases leaf ascorbate content. Proc Natl Acad Sci USA 104:9534鈥?539
  Letunic I, Copley RR, Schmidt S, Ciccarelli FD, Doerks T, Schultz J, Ponting CP, Bork P (2004) SMART 4.0: towards genomic data integration. Nucleic Acids Res 32:D142鈥揇144PubMed Central PubMed View Article
  Li Y, Schellhorn HE (2007) New developments and novel therapeutic perspectives for vitamin C. J Nutr 137:2171鈥?184PubMed
  Li Y, Song YP, Shi GJ, Wang JJ, Hou XL (2009) Response of antioxidant activity to excess copper in two cultivars of Brassica campestris ssp. chinensis Makino. Acta Physiol Plant 31:155鈥?62View Article
  Linster CL, Clarke SG (2008) l -Ascorbate biosynthesis in higher plants: the role of VTC2. Trends Plant Sci 13:567鈥?73PubMed Central PubMed View Article
  Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2-螖螖CT method. Methods 25:402鈥?08PubMed View Article
  Mandl J, Szarka A, B谩nhegyi G (2009) Vitamin C: update on physiology and pharmacology. Br J Pharmacol 157:1097鈥?110PubMed Central PubMed View Article
  Qin A, Shi Q, Yu X (2011) Ascorbic acid contents in transgenic potatoplants overexpressing two dehydroascorbate reductase genes. Mol Biol Rep 38:1557鈥?566PubMed View Article
  Ren J, Chen ZW, Duan WK, Song XM, Liu TK, Wang JJ, Hou XL, Li Y (2013) Comparis on of ascorbic acid biosynthesis in different tissues of three non-heading Chinese cabbage cultivars. Plant Physiol Biochem 73:229鈥?36PubMed View Article
  Sanmartin M, Drogoudi PD, Lyons T, Pateraki I, Barnes J, Kanellis AK (2003) Over-expression of ascorbate oxidase in the apoplast of transgenic tobacco results in altered ascorbate and glutathione redox states and increased sensitivity to ozone. Planta 216:918鈥?28PubMed
  Smirnoff N (2003) Ascorbic acid: metabolism and functions of a multi-facetted molecule. Curr Opin Plant Biol 3:229鈥?35View Article
  Stevens R, Page D, Gouble B, Garchery C, Zamir D, Causse M (2008) Tomato fruit ascorbic acid content is linked with monodehydroascorbate reductase activity and tolerance to chilling stress. Plant Cell Environ 31:1086鈥?096PubMed View Article
  Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731鈥?739PubMed Central PubMed View Article
  Tokunaga T, Miyahara K, Tabata K, Esaka M (2005) Generation and properties of ascorbic acid-overproducing transgenic tobacco cells expressing sense RNA for l -galactono-1,4-lactone dehydrogenase. Planta 220:854鈥?63PubMed View Article
  Veljovic-Jovanovic SD, Pignocchi C, Noctor G, Foyer CH (2001) Low ascorbic acid in the vtc-1 mutant of Arabidopsis is associated with decreased growth and intracellular redistribution of the antioxidant system. Plant Physiol 127:426鈥?35PubMed Central PubMed View Article
  Yang XY, Xie JX, Wang FF, Zhong J, Liu YZ, Li GH, Peng SA (2011) Comparison of ascorbate metabolism in fruits of two citrus species with obvious difference in ascorbate content in pulp. J Plant Physiol 168:2196鈥?205PubMed View Article
  Zechmann B, Stumpe M, Mauch F (2011) Immunocytochemical determination of the subcellular distribution of ascorbate in plants. Planta 233:1鈥?2PubMed Central PubMed View Article
  Zhang WY, Lorence A, Gruszewski HA, Chevone BI, Nessler CL (2009) AMR1, an Arabidopsis gene that coordinately and negatively regulates the Mannose/l -Galactose ascorbic acid biosynthetic pathway. Plant Physiol 150:942鈥?50PubMed Central PubMed View Article
  
• 作者单位：Jun Ren (1) (2) (3)
  Weike Duan (1) (2) (3)
  Zhongwen Chen (1) (2) (3)
  Shuo Zhang (1) (2) (3)
  Xiaoming Song (1) (2) (3)
  Tongkun Liu (1) (2) (3)
  Xilin Hou (1) (2) (3)
  Ying Li (1) (2) (3)
  
  1. Horticultural Department, Nanjing Agricultural University, Nanjing, 210095, China
  2. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing, 210095, China
  3. Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture, Nanjing, 210095, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1572-9818

文摘

l-Ascorbic acid is an important antioxidant in both animals and plants. Monodehydroascorbate reductase is an enzyme involved in regulating the l-ascorbic acid level in plants. Here, we report the cloning of the reductase gene from non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino) and negative regulation of l-ascorbic acid and dehydroascorbate levels in transgenic tobacco plants expressing the gene under the control of the 35S promoter. The transgenic plants had reduced growth. The transgenic plants had changed the expression of the genes in the d-mannose/l-galactose pathway, degradation and recycling pathway and the activity of enzymes involved in degradation (ascorbate oxidase and ascorbate peroxidase) or recycling of ascorbic acid (dehydroascorbate reductase and glutathione reductase).