Genotype variation and genetic relationship among Escherichia coli from nursery pigs located in different pens in the same farm
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- 作者：Ana Herrero-Fresno ; Shahana Ahmed ; Monica Hegstad Hansen…
- 关键词：E. coli ; Swine ; Genetic diversity ; Genetic relationship ; Antimicrobial selection ; REP ; PCR ; PFGE
- 刊名：BMC Microbiology
- 出版年：2017
- 出版时间：December 2017
- 年：2017
- 卷：17
- 期：1
- 全文大小：693KB
- 刊物主题：Microbiology; Biological Microscopy; Mycology; Parasitology; Virology; Life Sciences, general;
- 出版者：BioMed Central
- ISSN：1471-2180
- 卷排序：17
文摘
BackgroundSo far, little is known about the genetic diversity and relatedness among Escherichia coli (E. coli) populations in the gut of swine. Information on this is required to improve modeling studies on antimicrobial resistance aiming to fight its occurrence and development. This work evaluated the genotype variation of E. coli isolated from swine fecal samples at the single pig and pen level, as well as between pens using repetitive extragenic palindromic (REP) PCR fingerprinting and pulsed field gel electrophoresis (PFGE). The genetic diversity of strains collected from media supplemented with ampicillin or tetracycline was also investigated. Besides, the genetic relationship of strains within each pen, between pens, as well as among strains within each group isolated from media with or without antibiotic, was assessed.