Helicobacter pylori antibody patterns in Germany: a cross-sectional population study

详细信息    查看全文

• 作者：Angelika Michel ; Michael Pawlita ; Heiner Boeing ; Lutz Gissmann…
• 关键词：Helicobacter pylori ; Immune response ; Bead ; based multiplex serology
• 刊名：Gut Pathogens
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：6
• 期：1
• 全文大小：624 KB
• 参考文献：1. Parkin, DM (2006) The global health burden of infection-associated cancers in the year 2002. Int J Cancer 118: pp. 3030-3044 CrossRef
  2. Bruce, MG, Maaroos, HI (2008) Epidemiology of Helicobacter pylori infection. Helicobacter 13: pp. 1-6 CrossRef
  3. Perez-Perez, GI, Rothenbacher, D, Brenner, H (2004) Epidemiology of Helicobacter pylori infection. Helicobacter 9: pp. 1-6 CrossRef
  4. Weyermann, M, Rothenbacher, D, Brenner, H (2009) Acquisition of Helicobacter pylori infection in early childhood: independent contributions of infected mothers, fathers, and siblings. Am J Gastroenterol 104: pp. 182-189 CrossRef
  5. Rowland, M, Daly, L, Vaughan, M, Higgins, A, Bourke, B, Drumm, B (2006) Age-specific incidence of Helicobacter pylori. Gastroenterology 130: pp. 65-72 CrossRef
  6. Fox, JG, Wang, TC (2007) Inflammation, atrophy, and gastric cancer. J Clin Invest 117: pp. 60-69 CrossRef
  7. Roderick, P, Davies, R, Raftery, J, Crabbe, D, Pearce, R, Bhandari, P, Patel, P (2003) The cost-effectiveness of screening for Helicobacter pylori to reduce mortality and morbidity from gastric cancer and peptic ulcer disease: a discrete-event simulation model. Health Technol Assess 7: pp. 1-86
  8. Atherton, JC, Blaser, MJ (2009) Coadaptation of Helicobacter pylori and humans: ancient history, modern implications. J Clin Invest 119: pp. 2475-2487 CrossRef
  9. Portal-Celhay, C, Perez-Perez, GI (2006) Immune responses to Helicobacter pylori colonization: mechanisms and clinical outcomes. Clin Sci (Lond) 110: pp. 305-314 CrossRef
  10. van Amsterdam, K, van Vliet, AH, Kusters, JG, van der Ende, A (2006) Of microbe and man: determinants of Helicobacter pylori-related diseases. FEMS Microbiol Rev 30: pp. 131-156 CrossRef
  11. Baltrus, DA, Blaser, MJ, Guillemin, K (2009) Helicobacter pylori Genome Plasticity. Genome Dyn 6: pp. 75-90 CrossRef
  12. Michel, A, Waterboer, T, Kist, M, Pawlita, M (2009) Helicobacter pylori multiplex serology. Helicobacter 14: pp. 525-535 CrossRef
  13. Waterboer, T, Sehr, P, Michael, KM, Franceschi, S, Nieland, JD, Joos, TO, Templin, MF, Pawlita, M (2005) Multiplex human papillomavirus serology based on in situ-purified glutathione s-transferase fusion proteins. Clin Chem 51: pp. 1845-1853 CrossRef
  14. Sehr, P, Zumbach, K, Pawlita, M (2001) A generic capture ELISA for recombinant proteins fused to glutathione S-transferase: validation for HPV serology. J Immunol Methods 253: pp. 153-162 CrossRef
  15. Gao, L, Weck, MN, Michel, A, Pawlita, M, Brenner, H (2009) Association between chronic atrophic gastritis and serum antibodies to 15?Helicobacter pylori proteins measured by multiplex serology. Cancer Res 69: pp. 2973-2980 CrossRef
  16. Gao, L, Michel, A, Weck, MN, Arndt, V, Pawlita, M, Brenner, H (2009) Helicobacter pylori infection and gastric cancer risk: evaluation of 15 H. pylori proteins determined by novel multiplex serology. Cancer Res 69: pp. 6164-6170 CrossRef
  17. Song, H, Michel, A, Nyren, O, Ekstrom, AM, Pawlita, M, Ye, W (2013) A CagA-independent cluster of antigens related to the risk of noncardia gastric cancer: Associations between Heli
• 刊物主题：Gastroenterology; Medical Microbiology; Parasitology;
• 出版者：BioMed Central
• ISSN：1757-4749

文摘

Background Helicobacter pylori infection that is usually acquired in childhood and lasts for lifetime is mostly asymptomatic but associated with severe gastrointestinal disease including cancer. During chronic infection, the gastric mucosa is histologically changing. This forces H. pylori to permanent adaptation in its gastric habitat by expression of different proteins which might be reflected in distinctive antibody patterns. Methods To characterize dynamics of the immune response to H. pylori we analysed 1797 sera of a cross-sectional study representative for the German population (age range 1-2 years) with multiplex serology, a fluorescent bead-based antibody binding assay that allows simultaneous and quantitative detection of antibodies. Fifteen recombinant, affinity-purified H. pylori proteins (UreA, GroEL, Catalase, NapA, CagA, CagM, Cagδ, HP0231, VacA, HpaA, Cad, HyuA, Omp, HcpC and HP0305) were used as antigens. Results H. pylori seroprevalence (positivity for at least three antigens) was 48% and increased with age from 12% in children 65?years. Prevalences were highest (>83%) for Omp, VacA and GroEL. For 11 proteins, seroprevalence was higher in males than females (P--.05) from age 55 onwards. For all antigens, the median prevalence increase per age decade was stronger in males (8.4%, range 3.8-12.9%) than females (6.1%, range 3.4-10.8%). However, among seropositives the median number of antigens recognized increased from children 65?years stronger in females (P--.02). Antibody reactivities to GroEL, HyuA, CagM, Catalase, NapA and UreA also increased stronger in females (average 1.7-fold/decade, SD 0.5) than in males (1.5-fold/decade, SD 0.4). Conclusion H. pylori antibody response accumulates qualitatively and quantitatively with age. This may reflect a lifelong stimulation of the immune response by chronically active infection.