MALDI-Q-TOF mass spectrometric determination of gold and platinum in tissues using their diethyldithiocarbamate chelate complexes

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• 作者：Kayoko Minakata (1)
  Hideki Nozawa (1)
  Itaru Yamagishi (1)
  Kunio Gonmori (1)
  Masako Suzuki (2)
  Koutaro Hasegawa (1)
  Amin Wurita (1)
  Kanako Watanabe (1)
  Osamu Suzuki (1)
  
• 关键词：Gold ; Platinum ; Matrix ; assisted laser desorption ionization quadrupole time ; of ; flight mass spectrometry ; α ; Cyano ; 4 ; hydroxycinnamic acid ; Tissue
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2014
• 出版时间：February 2014
• 年：2014
• 卷：406
• 期：5
• 页码：1331-1338
• 全文大小：385 KB
• 作者单位：Kayoko Minakata (1)
  Hideki Nozawa (1)
  Itaru Yamagishi (1)
  Kunio Gonmori (1)
  Masako Suzuki (2)
  Koutaro Hasegawa (1)
  Amin Wurita (1)
  Kanako Watanabe (1)
  Osamu Suzuki (1)
  
  1. Department of Legal Medicine, Hamamatsu University School of Medicine, 1-20-1 Handayama, Hamamatsu, 431-3192, Japan
  2. Research Equipment Center, Hamamatsu University School of Medicine, 1-20-1 Handayama, Hamamatsu, 431-3192, Japan
  
• ISSN：1618-2650

文摘

A rapid determination method is presented for gold (Au3+) and platinum (Pt4+) in tissues using matrix-assisted laser desorption ionization quadrupole time-of-flight mass spectrometry (MALDI-Q-TOF-MS). Au and Pt ions in wet-ashed tissue solution were reacted with diethyldithiocarbamate (DDC), and the resulting chelate complex ions Au(DDC)2 + and Pt(DDC)3 + were detected by MALDI-Q-TOF-MS using α-cyano-4-hydroxycinnamic acid as a matrix. The limit of detection (LOD) was 0.8?ng/g tissue and the quantification range was 2-00?ng/g for Au, and the LOD was 6?ng/g tissue and the quantification range was 20-,000?ng/g for Pt. The Pt levels detected by MALDI-Q-TOF-MS in several tissues of a patient overdosed with cisplatin were nearly the same as those detected by flow-injection electrospray ionization mass spectrometry. The LODs of Au and Pt were 0.04?pg per well (sample spot) and 0.3?pg per well, respectively. To our knowledge, this is the first attempt to quantify Au3+ and Pt4+ ions in tissues by MALDI-Q-TOF-MS. A rapid determination method is presented for gold and platinum in tissues using matrix-assisted laser desorption ionization quadrupole time-of-flight mass spectrometry (MALDI-Q-TOF-MS). Au and Pt ions in wet-ashed tissue solution were reacted with diethyldithiocarbamate (DDC), and the resulted chelate complex ions Au(DDC)2 + and Pt(DDC)3 + were detected using α-cyano-4-hydroxycinnamic acid as a matrix.