A fluorine-doped tin oxide electrode modified with gold nanoparticles for electrochemiluminescent determination of hydrogen peroxide released by living cells

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• 作者：Man Li ; Hongfang Gao ; Xiaofei Wang ; Yufeng Wang ; Honglan Qi…
• 关键词：ECL ; FTO ; Gold nanoparticles ; Luminol ; Hydrogen peroxide ; Macrophage cells ; HeLa cells
• 刊名：Microchimica Acta
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：184
• 期：2
• 页码：603-610
• 全文大小：
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Nanochemistry; Nanotechnology; Characterization and Evaluation of Materials; Analytical Chemistry; Microengineering;
• 出版者：Springer Vienna
• ISSN：1436-5073
• 卷排序：184

文摘

An electrogenerated chemiluminescence (ECL) based method is described for the determination of hydrogen peroxide (H₂O₂) released by living cells. It is making use of a fluorine-doped tin oxide (FTO) electrode that was modified with gold nanoparticles (AuNPs). The AuNP-FTO was fabricated by electrodeposition of AuNP on the surface of the FTO electrode and characterized by scanning electron microscopy and electrochemical techniques. The AuNP-FTO exhibits a significant electrocatalytic activity towards luminol-based ECL system compared to a bare FTO. The calibration plot covers the 10 nM to 1 μM H₂O₂ concentration range, with a 8 nM detection limit (at a signal-to-noise ratio of 3). The method enabled (a) the monitoring of drug-stimulated production of H₂O₂ released by living cells including RAW 264.7 macrophage cells and HeLa cells; and (b) the determination of inhibitors of H₂O₂ secretion. The method, due to strong signal amplification by AuNPs, provides a viable tool for determination of H₂O₂ in biological systems.