MiR-519a functions as a tumor suppressor in glioma by targeting the oncogenic STAT3 pathway

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• 作者：Li Hong ; Liu Ya-wei ; Wang Hai ; Zhou Qiang ; Li Jun-jie…
• 刊名：Journal of Neuro-Oncology
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：128
• 期：1
• 页码：35-45
• 全文大小：11,008 KB
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Oncology
  
• 出版者：Springer Netherlands
• ISSN：1573-7373
• 卷排序：128

文摘

Glioblastoma (GBM) is among the most aggressive primary brain tumors, with a median survival rate of 12–15 months. MicroRNAs have been implicated in GBM development as oncogenes or tumor suppressors. In this study, we demonstrated that miR-519a expression was frequently downregulated in GBM specimens and cell lines, and that low-levels miR-519a expression significantly correlated with poor outcomes associated with GBM. Analysis of The Cancer Genome Atlas also demonstrated that low miR-519a expression can predict poor clinical outcomes in classical and proneural GBM subtypes. Functionally, re-expression of miR-519a effectively reduced GBM cell proliferation, migration, and invasion. Mechanistically, we confirmed that the signal transducer and activator of transcription 3 (STAT3) 3′-UTR was a putative target of miR-519a, and that re-expression of STAT3 abrogated miR-519a function in GBM cells. Furthermore, we found that STAT3 expression negatively correlated with that of miR-519a in human GBM tissues. These results elucidated the prognostic value and tumor-suppressor role of miR-519a in GBM and further suggested it as a potential therapeutic target for GBM treatment.KeywordsC19MCGlioblastomamiR-519aSTAT3