Sulfathiazole grafted PEG-PLL as pH-sensitive shielding system for cationic gene delivery

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• 作者：Jialiang Xia ; Jie Chen ; Lin Lin ; Zhaopei Guo ; Bing Han ; Hongyan Yang…
• 关键词：Gene delivery ; Shielding system ; pH sensitive ; Biomaterials ; Nanoparticles
• 刊名：Polymer Bulletin
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：73
• 期：12
• 页码：3503-3511
• 全文大小：776 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Polymer Sciences
  Characterization and Evaluation Materials
  Soft Matter and Complex Fluids
  Physical Chemistry
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1436-2449
• 卷排序：73

文摘

Polycationic gene carriers, mPEG-b-PLL-g-succinyl sulfathiazole (denoted as PPS), were synthesized and characterized as a shielding system for cationic gene delivery. DNA/PEI/PPS ternary complexes were prepared by shielding PPS to DNA/PEI polyplex solution. PPS can shield the surface charge of DNA/PEI polyplexes from positive (27.7 mV) to negative (−24.6 mV), and the acidic condition should trigger the disassembly of ternary complex. The gene transfection efficiency of DNA/PEI/PPS was lower than DNA/PEI at pH 7.4, but at pH 6.0, it recovered to the same level as DNA/PEI. CLSM results showed the same trend as transfection at different pH. The suitable pH-triggered shielding/deshielding ability estimated that PPS should be a potential shielding system to be used in in vivo gene delivery.