Clinical biomarkers of pulmonary carcinoid tumors in never smokers via profiling miRNA and target mRNA

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• 作者：Bo Deng (1) (2)
  Julian Molina (3)
  Marie C Aubry (4)
  Zhifu Sun (1)
  Liang Wang (5)
  Bruce W Eckloff (6)
  George Vasmatzis (7)
  Ming You (8)
  Eric D Wieben (9)
  Jin Jen (4)
  Dennis A Wigle (10)
  Ping Yang (1)
  
  1. Department of Health Sciences Research ; Mayo Clinic College of Medicine ; Rochester ; Minnesota ; USA
  2. Thoracic Surgery Department ; Institute of Surgery Research ; Daping Hospital ; Third Military Medical University ; Chongqing ; People鈥檚 Republic of China
  3. Department of Oncology ; Division of Medical Oncology ; Mayo Clinic College of Medicine ; Rochester ; Minnesota ; USA
  4. Department of Laboratory Medicine and Pathology ; Mayo Clinic College of Medicine ; Rochester ; Minnesota ; USA
  5. Department of Pathology ; Medical College of Wisconsin Cancer Center ; Milwaukee ; Wisconsin ; USA
  6. Medical Genome Facility ; Mayo Clinic College of Medicine ; Rochester ; Minnesota ; USA
  7. Department of Molecular Medicine ; Mayo Clinic College of Medicine ; Rochester ; Minnesota ; USA
  8. Department of Cancer Center and Toxicology ; Medical College of Wisconsin ; Milwaukee ; Wisconsin ; USA
  9. Department of Biochemistry and Molecular Biology ; Mayo Clinic College of Medicine ; Rochester ; Minnesota ; USA
  10. Department of Surgery ; Division of General Thoracic Surgery ; Mayo Clinic College of Medicine ; Rochester ; Minnesota ; USA
  
• 关键词：miRNA ; mRNA ; Carcinoid ; Survival
• 刊名：Cell & Bioscience
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：4
• 期：1
• 全文大小：450 KB
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• 刊物主题：Cell Biology; Microbiology;
• 出版者：BioMed Central
• ISSN：2045-3701

文摘

Background miRNAs play key regulatory roles in cellular pathological processes. We aimed to identify clinically meaningful biomarkers in pulmonary carcinoid tumors (PCTs), a member of neuroendocrine neoplasms, via profiling miRNAs and mRNAs. Results From the total of 1145 miRNAs, we obtained 16 and 17 miRNAs that showed positive and negative fold changes (FCs, tumors vs. normal tissues) in the top 1% differentially expressed miRNAs, respectively. We uncovered the target genes that were predicted by at least two prediction tools and overlapped by at least one-half of the top miRNAs, which yielded 44 genes (FC2), respectively. Higher expressions of CREB5, PTPRB and COL4A3 predicted favorable disease free survival (Hazard ratio: 0.03, 0.19 and 0.36; P value: 0.03, 0.03 and 0.08). Additionally, 79 mutated genes have been found in nine PCTs where TP53 was the only repeated mutation. Conclusion We identified that the expressions of three genes have clinical implications in PCTs. The biological functions of these biomarkers warrant further studies.