Phospholipase A/Acyltransferase enzyme activity of H-rev107 inhibits the H-RAS signaling pathway

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• 作者：Chun-Hua Wang (13)
  Rong-Yaun Shyu (8) (9)
  Chang-Chieh Wu (10)
  Tzung-Chieh Tsai (11)
  Lu-Kai Wang (12)
  Mao-Liang Chen (13)
  Shun-Yuan Jiang (13)
  Fu-Ming Tsai (13)
  
  13. Department of Research ; Taipei Tzuchi Hospital ; The Buddhist Tzuchi Medical Foundation ; New Taipei City ; Taiwan
  8. Department of Internal Medicine ; Taipei Tzuchi Hospital ; The Buddhist Tzuchi Medical Foundation ; New Taipei City ; Taiwan
  9. School of Medicine ; Tzu Chi University ; Hualien ; Taiwan
  10. Department of Surgery ; Tri-Service General Hospital ; National Defense Medical Center ; Taipei ; Taiwan
  11. Department of Microbiology ; Immunology and Biopharmaceuticals ; National Chiayi University ; Chiayi ; Taiwan
  12. Graduate Institute of Life Sciences ; National Defense Medical Center ; Taipei ; Taiwan
  
• 关键词：H ; rev107 ; HRASLS3 ; PLA2G16 ; H ; RAS ; Phospholipase A/acyltransferase ; Acyl ; biotin exchange assay
• 刊名：Journal of Biomedical Science
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：21
• 期：1
• 全文大小：563 KB
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• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Biomedicine
  
• 出版者：Springer Netherlands
• ISSN：1423-0127

文摘

Background H-rev107, also called HRASLS3 or PLA2G16, is a member of the HREV107 type II tumor suppressor gene family. Previous studies showed that H-rev107 exhibits phospholipase A/acyltransferase (PLA/AT) activity and downregulates H-RAS expression. However, the mode of action and the site of inhibition of H-RAS by H-rev107 are still unknown. Results Our results indicate that H-rev107 was co-precipitated with H-RAS and downregulated the levels of activated RAS (RAS-GTP) and ELK1-mediated transactivation in epidermal growth factor-stimulated and H-RAS-cotransfected HtTA cervical cancer cells. Furthermore, an acyl-biotin exchange assay demonstrated that H-rev107 reduced H-RAS palmitoylation. H-rev107 has been shown to be a PLA/AT that is involved in phospholipid metabolism. Treating cells with the PLA/AT inhibitor arachidonyl trifluoromethyl ketone (AACOCF3) or methyl arachidonyl fluorophosphate (MAFP) alleviated H-rev107-induced downregulation of the levels of acylated H-RAS. AACOCF3 and MAFP also increased activated RAS and ELK1-mediated transactivation in H-rev107-expressing HtTA cells following their treatment with epidermal growth factor. In contrast, treating cells with the acyl-protein thioesterase inhibitor palmostatin B enhanced H-rev107-mediated downregulation of acylated H-RAS in H-rev107-expressing cells. Palmostatin B had no effect on H-rev107-induced suppression of RAS-GTP levels or ELK1-mediated transactivation. These results suggest that H-rev107 decreases H-RAS activity through its PLA/AT activity to modulate H-RAS acylation. Conclusions We made the novel observation that H-rev107 decrease in the steady state levels of H-RAS palmitoylation through the phospholipase A/acyltransferase activity. H-rev107 is likely to suppress activation of the RAS signaling pathway by reducing the levels of palmitoylated H-RAS, which decreases the levels of GTP-bound H-RAS and also the activation of downstream molecules. Our study further suggests that the PLA/AT activity of H-rev107 may play an important role in H-rev107-mediated RAS suppression.