Identification of multiple small heat-shock protein genes in Plutella xylostella (L.) and their expression profiles in response to abiotic stresses

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• 作者：Xi’en Chen (1)
  Yalin Zhang (1)
  
• 关键词：Small heat ; shock proteins ; Expression profiles ; Abiotic stress responses ; Plutella xylostella (L.)
• 刊名：Cell Stress and Chaperones
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：20
• 期：1
• 页码：23-35
• 全文大小：1,649 KB
• 参考文献：1. Aevermann BD, Waters ER (2008) A comparative genomic analysis of the small heat shock proteins in / Caenorhabditis elegans and / briggsae. Genetica 133:307-19 CrossRef
  2. Basha E, Friedrich KL, Vierling E (2006) The N-terminal arm of small heat shock proteins is important for both chaperone activity and substrate specificity. J Biol Chem 281:39943-9952 CrossRef
  3. Basha E, O’Neill H, Vierling E (2012) Small heat shock proteins and α-crystallins: dynamic proteins with flexible functions. Trends Biochem Sci 37:106-17 CrossRef
  4. Basha E, Jones C, Blackwell AE, Cheng G, Waters ER, Samsel KA, Siddique M, Pett V, Wysocki V, Vierling E (2013) An unusual dimeric small heat shock protein provides insight into the mechanism of this class of chaperones. J Mol Biol 425:1683-696 CrossRef
  5. Colinet H, Lee SF, Hoffmann A (2010) Temporal expression of heat shock genes during cold stress and recovery from chill coma in adult / Drosophila melanogaster. FEBS J 277:174-85 CrossRef
  6. Concha C, Edman RM, Belikoff EJ, Schiemann AH, Carey B, Scott MJ (2012) Organization and expression of the Australian sheep blowfly ( / Lucilia cuprina) hsp23, hsp24, hsp70 and hsp83 genes. Insect Mol Biol 21:169-80 CrossRef
  7. Franck E, Madsen O, van Rheede T, Ricard G, Huynen MA, de Jong WW (2004) Evolutionary diversity of vertebrate small heat shock proteins. J Mol Evol 59:792-05 CrossRef
  8. Garczynski SF, Unruh TR, Guédot C, Neven LG (2011) Characterization of three transcripts encoding small heat shock proteins expressed in the codling moth, / Cydia pomonella (Lepidoptera: Tortricidae). Insect Sci 18:473-83 CrossRef
  9. Gehring WJ, Wehner R (1995) Heat shock protein synthesis and thermotolerance in / Cataglyphis, an ant from the Sahara desert. Proc Natl Acad Sci U S A 92:2994-998 CrossRef
  10. Gkouvitsas T, Kontogiannatos D, Kourti A (2008) Differential expression of two small Hsps during diapause in the corn stalk borer / Sesamia nonagrioides (Lef.). J Insect Physiol 54:1503-510 CrossRef
  11. Gu J, Huang LX, Shen Y, Huang LH, Feng QL (2012) Hsp70 and small Hsps are the major heat shock protein members involved in midgut metamorphosis in the common cutworm, / Spodoptera litura. Insect Mol Biol 5:535-43 CrossRef
  12. Haslbeck M (2002) sHsps and their role in the chaperone network. Cell Mol Life Sci 59:1649-657 CrossRef
  13. Haslbeck M, Franzmann T, Weinfurtner D, Buchner J (2005) Some like it hot: the structure and function of small heat-shock proteins. Nat Struct Mol Biol 12:842-46 CrossRef
  14. Hayward SAL, Pavlides SC, Tammariello SP, Rinehart JP, Denlinger DL (2005) Temporal expression patterns of diapause-associated genes in flesh fly pupae from the onset of diapause through post-diapause quiescence. J Insect Physiol 51:631-40 CrossRef
  15. Huang LH, Kang L (2007) Cloning and interspecific altered expression of heat shock protein genes in two leafminer species in response to thermal stress. Insect Mol Biol 16:491-00 CrossRef
  16. Huang LH, Wang CZ, Kang L (2009) Cloning and expression of five heat shock protein genes in relation to cold
• 作者单位：Xi’en Chen (1)
  Yalin Zhang (1)
  
  1. Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, College of Plant Protection, Northwest A & F University, Yangling, Shaanxi, China
  
• ISSN：1466-1268

文摘

We identify and characterize 14 small heat-shock protein (sHSP) genes from the diamondback moth (DBM), Plutella xylostella (L.), a destructive pest. Phylogenetic analyses indicate that, except for sHSP18.8 and sHSP19.22, the other 12 DBM sHSPs belong to five known insect sHSP groups. Developmental expression analysis revealed that most sHSPs peaked in the pupal and adult stages. The transcripts of sHSPs display tissue specificity with two exhibiting constitutive expression in four tested tissues. Expression of sHSP18.8 in fourth instar larvae is not induced by the tested abiotic stressors, and unless sHSP21.8 is not sensitive to thermal stress, 12 sHSPs are significantly up-regulated. The messenger RNA (mRNA) levels of all sHSPs are reduced under oxidative stress. Food deprivation leads to significant down-regulation of three sHSPs. The majority of sHSPs show expression variation to various heavy metals, whereas mRNA abundances of sHSP22.1 and sHSP 28.9 are reduced by four heavy metals. The responses of sHSPs to indoxacarb and cantharidin are varied. Beta-cypermethrin and chlorfenapyr exposure results in an increase of 13 sHSP transcripts and a reduction of 12 sHSP transcripts, respectively. These results show that different sHSPs might play distinct roles in the development and regulation of physiological activities, as well as in response to various abiotic stresses of DBM.