Glycogen synthase kinase 3 (GSK3)-inhibitor SB216763 promotes the conversion of human umbilical cord mesenchymal stem cells into neural precursors in adherent culture
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- 作者：Liyang Gao ; Mingyan Zhao ; Peng Li ; Junchao Kong ; Zhijun Liu ; Yonghua Chen…
- 关键词：Human umbilical cord mesenchymal stem cells ; GSK3 inhibitor ; Neural differentiation ; Neural precursor cells ; N2 supplement ; Default mechanism
- 刊名：Human Cell
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：30
- 期：1
- 页码：11-22
- 全文大小：
- 刊物主题：Cell Biology; Embryology; Oncology; Stem Cells; Reproductive Medicine; Cell Culture;
- 出版者：Springer Japan
- ISSN：1749-0774
- 卷排序：30
文摘
The ability to generate neural progenitor cells from human umbilical cord mesenchymal stem cells (hUC-MSCs) has provided an option to treat neurodegenerative diseases. To establish a method for this purpose, we characterized the early neural markers of hUC-MSCs-derived cells under different conditions. We found that neither the elimination of signals for alternative fate nor N2 supplement was sufficient to differentiate hUC-MSCs into neural precursor cells, but the GSK3 inhibitor SB216763 could promote an efficient neural commitment of hUC-MSCs. The results indicated that Wnt/β-catenin might play an important role during the early neural differentiation of hUC-MSCs. Here, we report a method for hUC-MSCs to commit efficiently into a neural fate within a short period of time. This protocol provides an efficient method for hUC-MSCs-based neural regeneration.