Circulating miR-18a in plasma contributes to cancer detection and monitoring in patients with gastric cancer

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• 作者：Masahiro Tsujiura ; Shuhei Komatsu ; Daisuke Ichikawa ; Atsushi Shiozaki…
• 关键词：Biomarker ; Circulating nucleic acids ; Gastric cancer ; microRNA ; Plasma
• 刊名：Gastric Cancer
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：18
• 期：2
• 页码：271-279
• 全文大小：1,059 KB
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  19. He, L, Thoms
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Oncology
  Gastroenterology
  Surgical Oncology
  Pathology
  Radiotherapy
  
• 出版者：Springer Japan
• ISSN：1436-3305

文摘

Background Recently, circulating microRNAs have been reported to be stably detectable in plasma/serum and to function as potent non-invasive biomarkers in various cancers. We hypothesized that miR-18a could contribute to a novel plasma biomarker in patients with gastric cancer (GC). Methods We focused on miR-18a, which is a component of miR-17-92 cluster and has been reported as highly expressed in GC tissues. The study involved three steps: (1) confirmation of the higher miR-18a expression in primary GC tissues and GC cell lines than in normal gastric tissues and a fibroblast cell line; (2) evaluation of the plasma miR-18a assay using quantitative RT-PCR by comparing 104 GC patients and 65 healthy volunteers; (3) evaluation of monitoring tumor dynamics by the plasma miR-18a assay. Results (1) The miR-18a expressions were significantly higher in GC tissues than in normal gastric tissues (P?=?0.0286) and higher in all examined GC cell lines than in the fibroblast cell line. (2) The plasma miR-18a concentrations were significantly higher in GC patients than in healthy controls (P?P?=?0.0002). In an miR-18a overexpressing cell line, the miR-18a concentration of cultured medium increased in both cell number and time-course dependent manners, suggesting microRNA might be released from cancer cells into the surrounding environment. Conclusions Circulating miR-18a could be a useful biomarker for screening GC and monitoring tumor dynamics.