Generation of mesenchymal stromal cells from cord blood: evaluation of in vitro quality parameters prior to clinical use

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• 作者：Eliana Amati ; Sabrina Sella ; Omar Perbellini…
• 刊名：Stem Cell Research & Therapy
• 出版年：2017
• 出版时间：December 2017
• 年：2017
• 卷：8
• 期：1
• 全文大小：1527KB
• 刊物主题：Stem Cells; Cell Biology;
• 出版者：BioMed Central
• ISSN：1757-6512
• 卷排序：8

文摘

BackgroundIncreasing evidence suggests the safety and efficacy of mesenchymal stromal cells (MSC) as advanced therapy medicinal products because of their immunomodulatory properties and supportive role in hematopoiesis. Although bone marrow remains the most common source for obtaining off-the-shelf MSC, cord blood (CB) represents an alternative source, which can be collected noninvasively and without major ethical concerns. However, the low estimated frequency and inconsistency of successful isolation represent open challenges for the use of CB-derived MSC in clinical trials. This study explores whether CB may represent a suitable source of MSC for clinical use and analyzes several in vitro parameters useful to better define the quality of CB-derived MSC prior to clinical application.