Genetic Manipulations in Dermatophytes
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- 作者：Mohamed Mahdi Alshahni ; Tsuyoshi Yamada
- 关键词：Dermatophytes ; Genetic manipulation ; Copper ; repressible promoter ; Marker recycling ; Homologous recombination ; Genetic transformation ; RNA interference
- 刊名：Mycopathologia
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：182
- 期：1-2
- 页码：33-43
- 全文大小：574KB
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Microbiology; Medical Microbiology; Eukaryotic Microbiology; Microbial Ecology; Plant Sciences;
- 出版者：Springer Netherlands
- ISSN：1573-0832
- 卷排序：182
文摘
Dermatophytes are a group of closely related fungi that nourish on keratinized materials for their survival. They infect stratum corneum, nails, and hair of human and animals, accounting the largest portion of fungi causing superficial mycoses. Huge populations are suffering from dermatophytoses, though the biology of these fungi is largely unknown yet. Reasons are partially attributed to the poor amenability of dermatophytes to genetic manipulation. However, advancements in this field over the last decade made it possible to conduct genetic studies to satisfying extents. These included genetic transformation methods, indispensable molecular tools, i.e., dominant selectable markers, inducible promoter, and marker recycling system, along with improving homologous recombination frequency and gene silencing. Furthermore, annotated genome sequences of several dermatophytic species have recently been available, ensuring an optimal recruitment of the molecular tools to expand our knowledge on these fungi. In conclusion, the establishment of basic molecular tools and the availability of genomic data will open a new era that might change our understanding on the biology and pathogenicity of this fungal group.