Hibiscus latent Fort Pierce virus in Brazil and synthesis of its biologically active full-length cDNA clone

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• 作者：Ruimin Gao ; Shengniao Niu ; Weifang Dai ; Elliot Kitajima ; Sek-Man Wong
• 关键词：Hibiscuslatent Fort Pierce virus ; Brazil ; Biologically active HLFPV cDNA clone ; In vitro transcripts
• 刊名：Virus Genes
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：52
• 期：5
• 页码：754-757
• 全文大小：1,401 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Medical Microbiology
  Virology
  Plant Sciences
  
• 出版者：Springer Netherlands
• ISSN：1572-994X
• 卷排序：52

文摘

A Brazilian isolate of Hibiscus latent Fort Pierce virus (HLFPV-BR) was firstly found in a hibiscus plant in Limeira, SP, Brazil. RACE PCR was carried out to obtain the full-length sequences of HLFPV-BR which is 6453 nucleotides and has more than 99.15 % of complete genomic RNA nucleotide sequence identity with that of HLFPV Japanese isolate. The genomic structure of HLFPV-BR is similar to other tobamoviruses. It includes a 5′ untranslated region (UTR), followed by open reading frames encoding for a 128-kDa protein and a 188-kDa readthrough protein, a 38-kDa movement protein, 18-kDa coat protein, and a 3′ UTR. Interestingly, the unique feature of poly(A) tract is also found within its 3′-UTR. Furthermore, from the total RNA extracted from the local lesions of HLFPV-BR-infected Chenopodium quinoa leaves, a biologically active, full-length cDNA clone encompassing the genome of HLFPV-BR was amplified and placed adjacent to a T7 RNA polymerase promoter. The capped in vitro transcripts from the cloned cDNA were infectious when mechanically inoculated into C. quinoa and Nicotiana benthamiana plants. This is the first report of the presence of an isolate of HLFPV in Brazil and the successful synthesis of a biologically active HLFPV-BR full-length cDNA clone.