Effect of cryopreservation on delineation of immune cell subpopulations in tumor specimens as determined by multiparametric single cell mass cytometry analysis

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• 作者：Elma Kadić ; Raymond J. Moniz ; Ying Huo ; An Chi ; Ilona Kariv
• 关键词：Mass Cytometry (MC) ; CyTOF™Flow cytometry (FC) ; Tumor samples ; Tumor microenvironment (TME) ; Tumor infiltrating lymphocytes (TILs) ; Immunophenotyping ; Cryopreservation ; ViSNE
• 刊名：BMC Immunology
• 出版年：2017
• 出版时间：December 2017
• 年：2017
• 卷：18
• 期：1
• 全文大小：2230KB
• 刊物主题：Immunology; Allergology; Vaccine; Cytokines and Growth Factors;
• 出版者：BioMed Central
• ISSN：1471-2172
• 卷排序：18

文摘

BackgroundComprehensive understanding of cellular immune subsets involved in regulation of tumor progression is central to the development of cancer immunotherapies. Single cell immunophenotyping has historically been accomplished by flow cytometry (FC) analysis, enabling the analysis of up to 18 markers. Recent advancements in mass cytometry (MC) have facilitated detection of over 50 markers, utilizing high resolving power of mass spectrometry (MS). This study examined an analytical and operational feasibility of MC for an in-depth immunophenotyping analysis of the tumor microenvironment, using the commercial CyTOF™ instrument, and further interrogated challenges in managing the integrity of tumor specimens.