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Immobilization of uricase-gold nanoparticles composite nanomaterial on a biofilm and its application to determination of uric acid
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  • 作者:Y. Zhang ; M. Yan ; P. Gao ; J. Jiang ; G. Zhang…
  • 关键词:gold nanoparticles ; uricase ; biofilm ; uric acid ; biosensors
  • 刊名:Applied Biochemistry and Microbiology
  • 出版年:2015
  • 出版时间:July 2015
  • 年:2015
  • 卷:51
  • 期:4
  • 页码:470-478
  • 全文大小:1,217 KB
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  • 作者单位:Y. Zhang (1)
    M. Yan (1)
    P. Gao (1)
    J. Jiang (1)
    G. Zhang (1)
    J. Li (1)
    S. Shuang (1)

    1. School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan, 030006, PR China
  • 刊物类别:Biomedical and Life Sciences
  • 刊物主题:Life Sciences
    Biochemistry
    Microbiology
    Medical Microbiology
    Russian Library of Science
  • 出版者:MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
  • ISSN:1608-3024
文摘
The uricase-gold nanoparticles (AuNPs) composite nanomaterial was covalently immobilized on a biofilm, eggshell membrane. The uric acid biosensor was constructed by positioning the immobilized biofilm on the surface of a Clark dissolved oxygen electrode. The scanning electron micrograph revealed that the uricase-AuNPs composite nanomaterial was successfully immobilized. The effects of enzyme loading, pH, concentration of phosphate buffer and temperature on the biosensor response have been studied in detail. The results showed that response time of the biosensor was 60 s, the linear range for the detection of uric acid varied from 1.0 μM to ~1.0 mM with a detection limit of 0.8 μM (S/N = 3) and relative standard deviation of 2.6% for 0.1 mM uric acid (n = 6). Some common potential interferents in samples demonstrated no interferences. The biosensor has been successfully applied to determine the uric acid level in urine and serum samples.

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