Regeneration of fertile, hexaploid, interspecific hybrids of elephantgrass and pearl millet following treatment of embryogenic calli with antimitotic agents

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• 作者：F谩bio G. Faleiro ; Baskaran Kannan…
• 关键词：Elephantgrass ; Chromosome doubling ; Tissue culture ; Oryzalin ; Trifluralin ; Stomata size ; Flow cytometry ; Biofuel feedstock ; Forage
• 刊名：Plant Cell, Tissue and Organ Culture
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：124
• 期：1
• 页码：57-67
• 全文大小：1,238 KB
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• 作者单位：F谩bio G. Faleiro (1) (4)
  Baskaran Kannan (1)
  Fredy Altpeter (1) (2) (3)
  
  1. Agronomy Department, University of Florida, IFAS, Gainesville, FL, USA
  4. Embrapa Cerrados, P.O. Box 08223, 73310-970, Planaltina, DF, Brazil
  2. Plant Molecular and Cellular Biology Program, University of Florida, IFAS, Gainesville, FL, USA
  3. University of Florida Genetics Institute, Gainesville, FL, USA
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1573-5044

文摘

Elephantgrass (Pennisetum purpureum, 2n = 4x = 28) produces large amounts of biomass in tropical and subtropical regions and is considered a prime candidate for lignocellulosic biofuel production. Interspecific hybridization between elephantgrass and pearl millet (Pennisetum glaucum, 2n = 2x = 14) may allow improvement of drought tolerance and biomass quality. These interspecific hybrids are male and female sterile due to their triploid genome (2n = 3x = 21). Chromosome doubling of the triploid hybrids may restore fertility, permitting a backcross with the recurrent or other elephantgrass parents to enhance biomass yield and persistence. In this study, chromosome doubling of productive interspecific hybrids was performed in vitro. Immature inflorescence cross-sections of five interspecific hybrids with good agronomic performance were used as explants for establishment of embryogenic tissue cultures. These calli were treated with different antimitotic agents, oryzalin or trifluralin. Chromosome doubling was achieved in all five interspecific hybrids and a total of 74 plants with altered ploidy were confirmed by flow cytometry. Stomatal size determination was a suitable screening tool for identification of hexaploid events. Genotypes MN18 and MN51 had the highest number of plants with altered ploidy of 29 and 27, respectively. Oryzalin at 5 碌M was the most effective antimitotic treatment and produced 55 of the 74 plants with altered ploidy. The most vigorous lines were grown to maturity and produced viable pollen. Keywords Elephantgrass Chromosome doubling Tissue culture Oryzalin Trifluralin Stomata size Flow cytometry Biofuel feedstock Forage