In vivo estimation of optical properties of rat liver using single-reflectance fiber probe during ischemia and reperfusion

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• 作者：Sharmin Akter ; Tomoki Tanabe ; Satoshi Maejima ; Satoko Kawauchi…
• 关键词：Diffuse reflectance spectroscopy ; Absorption coefficient ; Reduced scattering coefficient ; Tissue oxygen saturation ; Cytochrome c oxidase ; Liver ischemia–reperfusion
• 刊名：Optical Review
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：23
• 期：2
• 页码：354-359
• 全文大小：503 KB
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• 作者单位：Sharmin Akter (1)
  Tomoki Tanabe (1)
  Satoshi Maejima (2)
  Satoko Kawauchi (3)
  Shunichi Sato (3)
  Akinari Hinoki (2)
  Suefumi Aosasa (2)
  Junji Yamamoto (2)
  Izumi Nishidate (1)
  
  1. Graduate School of Bio-Application and Systems Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan
  2. Department of Surgery, National Defense Medical College, Tokorozawa, Saitama, 359-8513, Japan
  3. Division of Biomedical Information Sciences, National Defense Medical College Research Institute, Tokorozawa, Saitama, 359-8513, Japan
  
• 刊物类别：Physics and Astronomy
• 刊物主题：Physics
  Electromagnetism, Optics and Lasers
  
• 出版者：The Optical Society of Japan, co-published with Springer-Verlag GmbH
• ISSN：1349-9432

文摘

To quantify the changes in optical properties of in vivo rat liver tissue, we applied diffuse reflectance spectroscopy (DRS) system using single-reflectance fiber probe during ischemia and reperfusion evoked by hepatic portal occlusion (hepatic artery, portal vein and bile duct). Changes in the reduced scattering coefficient μ _s′, the absorption coefficient μ _a, the tissue oxygen saturation StO₂, and the oxidation of heme aa3 in cytochrome c oxidase (CcO) OHaa₃ of in vivo rat liver (n = 6) were evaluated. Heme aa₃ in CcO were significantly reduced (P < 0.05) during ischemia, which indicates a sign of mitochondrial energy failure induced by oxygen insufficiency of liver tissue. We found that OHaa₃ obtained from the proposed method was unchanged immediately after the onset of ischemia and started gradually decreasing at 2 min after the onset of ischemia. Difference in the time course between OHaa₃ and the conventional ratio metric analysis with μ _a(605)/μ _a(620) reported in literature demonstrates that the proposed method is effective in reduction of optical cross talk between hemoglobin and heme aa₃. Our results suggest that DRS technique is applicable and useful for assessing in vivo tissue viability and hemodynamics in liver intraoperatively.